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ENCSR853PBF

ENCSR ENCODE
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shRNA knockdown against DHX30 in HepG2 cells followed by RNA-seq. (DHX30-32-BGHLV20)

Organism: Homo sapiens
Experiment Types:
shRNA RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

shRNA RNA-seq of DHX30 in Homo sapiens HepG2 expressing RNAi targeting H. sapiens DHX30

Overall Design

shRNA knockdown against DHX30 in HepG2 cells followed by RNA-seq. (DHX30-32-BGHLV20)

Analysis page is available for ENCSR853PBF.

Dataset Citations (3)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 87.4 GB
Assay: shRNA RNA-seq
Target: DHX30
Biosample: Homo sapiens HepG2 expressing RNAi targeting H. sapiens DHX30
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF031KTB ENCFF031KTB.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 4.1 GB link
ENCFF519CYF ENCFF519CYF.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 4.2 GB link
ENCFF789AYA ENCFF789AYA.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.5 GB link
ENCFF872LKC ENCFF872LKC.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.6 GB link
ENCFF333CLM ENCFF333CLM.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.2 GB link
ENCFF720KPV ENCFF720KPV.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.1 GB link
ENCFF180JBY ENCFF180JBY.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.6 GB link
ENCFF402UGA ENCFF402UGA.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 4.1 GB link
ENCFF445RWU ENCFF445RWU.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.6 GB link
ENCFF502YPG ENCFF502YPG.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.5 GB link
ENCFF751TAL ENCFF751TAL.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 4.0 GB link
ENCFF815FBV ENCFF815FBV.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.5 GB link
ENCFF596KGB ENCFF596KGB.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 4.7 GB link
ENCFF602DTY ENCFF602DTY.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 4.8 GB link
ENCFF515ANL ENCFF515ANL.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.3 GB link
ENCFF625UXQ ENCFF625UXQ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.2 GB link
ENCFF329MVS ENCFF329MVS.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 4.7 GB link
ENCFF542NBD ENCFF542NBD.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 4.8 GB link
ENCFF020DQO ENCFF020DQO.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 74.4 MB link
ENCFF226OZA ENCFF226OZA.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 64.1 MB link
ENCFF348GGG ENCFF348GGG.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 64.4 MB link
ENCFF367GWJ ENCFF367GWJ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 72.6 MB link
ENCFF712AIB ENCFF712AIB.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 74.4 MB link
ENCFF815MJC ENCFF815MJC.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 72.7 MB link
ENCFF307BQZ ENCFF307BQZ.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 60.4 MB link
ENCFF390RNM ENCFF390RNM.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 73.3 MB link
ENCFF444YTI ENCFF444YTI.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 61.7 MB link
ENCFF671FCA ENCFF671FCA.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 65.0 MB link
ENCFF675QNI ENCFF675QNI.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 72.2 MB link
ENCFF680YNJ ENCFF680YNJ.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 65.0 MB link
ENCFF017YMK ENCFF017YMK.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 61.2 MB link
ENCFF028QEE ENCFF028QEE.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 65.2 MB link
ENCFF344GWG ENCFF344GWG.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 60.4 MB link
ENCFF682WDF ENCFF682WDF.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 66.8 MB link
ENCFF504LFH ENCFF504LFH.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 66.2 MB link
ENCFF770DKI ENCFF770DKI.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 65.2 MB link
ENCFF400ODE ENCFF400ODE.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 61.2 MB link
ENCFF466XVS ENCFF466XVS.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 57.6 MB link
ENCFF655WUY ENCFF655WUY.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 56.4 MB link
ENCFF702IKL ENCFF702IKL.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 60.3 MB link
ENCFF740ENI ENCFF740ENI.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 66.2 MB link
ENCFF910TAE ENCFF910TAE.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 65.9 MB link
ENCFF363OSS ENCFF363OSS.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 65.3 MB link
ENCFF459GGD ENCFF459GGD.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 75.7 MB link
ENCFF731HOS ENCFF731HOS.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 73.9 MB link
ENCFF751GDU ENCFF751GDU.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 65.5 MB link
ENCFF759QWF ENCFF759QWF.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 74.0 MB link
ENCFF921RKL ENCFF921RKL.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 75.7 MB link
ENCFF206DXD ENCFF206DXD.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 63.0 MB link
ENCFF335ZYN ENCFF335ZYN.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 66.3 MB link
ENCFF781IXU ENCFF781IXU.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 74.5 MB link
ENCFF834PAK ENCFF834PAK.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 61.9 MB link
ENCFF943FTA ENCFF943FTA.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 66.4 MB link
ENCFF983DCE ENCFF983DCE.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 73.3 MB link
ENCFF252KYY ENCFF252KYY.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 62.0 MB link
ENCFF741ZAB ENCFF741ZAB.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 62.7 MB link
ENCFF824BOB ENCFF824BOB.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 67.0 MB link
ENCFF993OBZ ENCFF993OBZ.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 68.5 MB link
ENCFF225TJN ENCFF225TJN.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 66.9 MB link
ENCFF712DJZ ENCFF712DJZ.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 68.0 MB link
ENCFF081CGD ENCFF081CGD.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 68.0 MB link
ENCFF208KSB ENCFF208KSB.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 62.0 MB link
ENCFF512APM ENCFF512APM.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 67.8 MB link
ENCFF524PCS ENCFF524PCS.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 58.3 MB link
ENCFF886YVP ENCFF886YVP.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 59.4 MB link
ENCFF891WXU ENCFF891WXU.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 62.9 MB link
ENCFF209LFW ENCFF209LFW.fastq fastq (reads) reads 2.1 GB link
ENCFF515OEI ENCFF515OEI.fastq fastq (reads) reads 2.3 GB link
ENCFF662EPC ENCFF662EPC.fastq fastq (reads) reads 2.1 GB link
ENCFF942ELT ENCFF942ELT.fastq fastq (reads) reads 2.2 GB link
ENCFF094XSH ENCFF094XSH.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 14.5 MB link
ENCFF485PKI ENCFF485PKI.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 3.2 MB link
ENCFF835ZMU ENCFF835ZMU.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 2.7 MB link
ENCFF180ZCX ENCFF180ZCX.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 2.9 MB link
ENCFF193LZV ENCFF193LZV.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.9 MB link
ENCFF246ZVR ENCFF246ZVR.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 4.2 MB link
ENCFF678BAP ENCFF678BAP.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 5.6 MB link
ENCFF918LAR ENCFF918LAR.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.8 MB link
ENCFF441NTZ ENCFF441NTZ.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF919YHL ENCFF919YHL.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF851FTW ENCFF851FTW.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF950STI ENCFF950STI.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF266BXR ENCFF266BXR.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF835QUB ENCFF835QUB.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF180VNG ENCFF180VNG.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF546UVR ENCFF546UVR.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF092MUL ENCFF092MUL.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF433WGE ENCFF433WGE.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF458EWR ENCFF458EWR.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF596FJS ENCFF596FJS.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF045TOT ENCFF045TOT.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link
ENCFF580TXE ENCFF580TXE.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link