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ENCSR147ZBD

ENCSR ENCODE
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shRNA knockdown against DDX6 in HepG2 cells followed by RNA-seq. (DDX6-BGHLV12)

Organism: Homo sapiens
Experiment Types:
shRNA RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

shRNA RNA-seq of DDX6 in Homo sapiens HepG2 expressing RNAi targeting H. sapiens DDX6

Overall Design

shRNA knockdown against DDX6 in HepG2 cells followed by RNA-seq. (DDX6-BGHLV12)

Analysis page is available for ENCSR147ZBD.

Dataset Citations (3)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 143.7 GB
Assay: shRNA RNA-seq
Target: DDX6
Biosample: Homo sapiens HepG2 expressing RNAi targeting H. sapiens DDX6
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF226GMB ENCFF226GMB.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 6.9 GB link
ENCFF368UHA ENCFF368UHA.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 5.7 GB link
ENCFF663NFQ ENCFF663NFQ.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 5.9 GB link
ENCFF870ZRZ ENCFF870ZRZ.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 7.3 GB link
ENCFF258BON ENCFF258BON.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 6.9 GB link
ENCFF997EMY ENCFF997EMY.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 7.3 GB link
ENCFF343WSX ENCFF343WSX.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 5.0 GB link
ENCFF365JNK ENCFF365JNK.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 5.9 GB link
ENCFF401NSP ENCFF401NSP.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 5.4 GB link
ENCFF646CIH ENCFF646CIH.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 5.0 GB link
ENCFF655VZR ENCFF655VZR.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 5.3 GB link
ENCFF725RWM ENCFF725RWM.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 6.3 GB link
ENCFF743XZQ ENCFF743XZQ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 8.9 GB link
ENCFF779HXE ENCFF779HXE.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 8.0 GB link
ENCFF200TBC ENCFF200TBC.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 10.0 GB link
ENCFF669KAR ENCFF669KAR.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 9.0 GB link
ENCFF082EAB ENCFF082EAB.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 8.9 GB link
ENCFF121CEF ENCFF121CEF.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 8.0 GB link
ENCFF019OMW ENCFF019OMW.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 75.7 MB link
ENCFF296KAJ ENCFF296KAJ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 80.4 MB link
ENCFF929GAD ENCFF929GAD.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 73.4 MB link
ENCFF993BJV ENCFF993BJV.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 84.0 MB link
ENCFF438BTX ENCFF438BTX.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 80.5 MB link
ENCFF591CKM ENCFF591CKM.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 84.0 MB link
ENCFF128DXO ENCFF128DXO.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 75.3 MB link
ENCFF230PIZ ENCFF230PIZ.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 80.2 MB link
ENCFF320ZRE ENCFF320ZRE.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 71.5 MB link
ENCFF489UAC ENCFF489UAC.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 74.2 MB link
ENCFF545WQN ENCFF545WQN.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 83.4 MB link
ENCFF751YMD ENCFF751YMD.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 77.1 MB link
ENCFF094RFX ENCFF094RFX.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 73.0 MB link
ENCFF352EGL ENCFF352EGL.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 76.1 MB link
ENCFF629RCD ENCFF629RCD.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 71.6 MB link
ENCFF769QHL ENCFF769QHL.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 68.7 MB link
ENCFF216IFC ENCFF216IFC.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 73.1 MB link
ENCFF637YXM ENCFF637YXM.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 76.0 MB link
ENCFF066XSC ENCFF066XSC.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 69.5 MB link
ENCFF253HRN ENCFF253HRN.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 72.4 MB link
ENCFF477NKX ENCFF477NKX.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 76.1 MB link
ENCFF662YIX ENCFF662YIX.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 71.3 MB link
ENCFF781RXU ENCFF781RXU.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 73.1 MB link
ENCFF945RWA ENCFF945RWA.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 67.6 MB link
ENCFF085LXQ ENCFF085LXQ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 74.6 MB link
ENCFF166QBX ENCFF166QBX.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 81.8 MB link
ENCFF388PFU ENCFF388PFU.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 77.1 MB link
ENCFF649VHQ ENCFF649VHQ.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 85.6 MB link
ENCFF041PVO ENCFF041PVO.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 81.8 MB link
ENCFF970BMN ENCFF970BMN.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 85.7 MB link
ENCFF147CKI ENCFF147CKI.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 72.8 MB link
ENCFF582RSQ ENCFF582RSQ.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 84.9 MB link
ENCFF666CEY ENCFF666CEY.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 81.4 MB link
ENCFF865SBX ENCFF865SBX.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 78.6 MB link
ENCFF877XXF ENCFF877XXF.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 77.0 MB link
ENCFF892VYQ ENCFF892VYQ.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 75.6 MB link
ENCFF186FQN ENCFF186FQN.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 70.2 MB link
ENCFF203QKE ENCFF203QKE.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 73.3 MB link
ENCFF617DGF ENCFF617DGF.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 78.1 MB link
ENCFF819OAN ENCFF819OAN.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 74.7 MB link
ENCFF328GLT ENCFF328GLT.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 74.8 MB link
ENCFF970VDT ENCFF970VDT.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 77.9 MB link
ENCFF139XIT ENCFF139XIT.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 78.1 MB link
ENCFF247IQI ENCFF247IQI.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 74.3 MB link
ENCFF261PBC ENCFF261PBC.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 71.2 MB link
ENCFF599QIG ENCFF599QIG.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 73.1 MB link
ENCFF778QHP ENCFF778QHP.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 69.4 MB link
ENCFF872RKD ENCFF872RKD.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 74.9 MB link
ENCFF353TCN ENCFF353TCN.fastq fastq (reads) reads 3.6 GB link
ENCFF639SIY ENCFF639SIY.fastq fastq (reads) reads 3.4 GB link
ENCFF697HUP ENCFF697HUP.fastq fastq (reads) reads 3.3 GB link
ENCFF897ZIG ENCFF897ZIG.fastq fastq (reads) reads 3.6 GB link
ENCFF176NQP ENCFF176NQP.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 3.3 MB link
ENCFF476XIK ENCFF476XIK.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 4.0 MB link
ENCFF751ALB ENCFF751ALB.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 16.6 MB link
ENCFF151NTA ENCFF151NTA.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.7 MB link
ENCFF288ITA ENCFF288ITA.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 2.0 MB link
ENCFF354QLG ENCFF354QLG.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 3.0 MB link
ENCFF752WJO ENCFF752WJO.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 5.5 MB link
ENCFF850KXY ENCFF850KXY.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 4.4 MB link
ENCFF037YBM ENCFF037YBM.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.5 MB link
ENCFF447TZY ENCFF447TZY.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.5 MB link
ENCFF008AWY ENCFF008AWY.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF992NSK ENCFF992NSK.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF533HCH ENCFF533HCH.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF649NBU ENCFF649NBU.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF727ZQB ENCFF727ZQB.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF937TOO ENCFF937TOO.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF120GTP ENCFF120GTP.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF208QZF ENCFF208QZF.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.3 MB link
ENCFF220YEM ENCFF220YEM.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF397CIQ ENCFF397CIQ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF104ZCF ENCFF104ZCF.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link
ENCFF558LQM ENCFF558LQM.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link