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ENCSR546MBH

ENCSR ENCODE
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shRNA knockdown against EIF2S1 in K562 cells followed by RNA-seq. (EIF2S1-LV08)

Organism: Homo sapiens
Experiment Types:
shRNA RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

shRNA RNA-seq of EIF2S1 in Homo sapiens K562 expressing RNAi targeting H. sapiens EIF2S1

Overall Design

shRNA knockdown against EIF2S1 in K562 cells followed by RNA-seq. (EIF2S1-LV08)

Analysis page is available for ENCSR546MBH.

Dataset Citations (3)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 105.2 GB
Assay: shRNA RNA-seq
Target: EIF2S1
Biosample: Homo sapiens K562 expressing RNAi targeting H. sapiens EIF2S1
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF064SEH ENCFF064SEH.bam bam (alignments) alignments 3.9 GB link
ENCFF071ACA ENCFF071ACA.bam bam (alignments) alignments 4.9 GB link
ENCFF088KZD ENCFF088KZD.bam bam (alignments) alignments 4.7 GB link
ENCFF121ZAU ENCFF121ZAU.bam bam (alignments) alignments 4.4 GB link
ENCFF308QLD ENCFF308QLD.bam bam (alignments) alignments 5.2 GB link
ENCFF345BNB ENCFF345BNB.bam bam (alignments) alignments 4.9 GB link
ENCFF444SWH ENCFF444SWH.bam bam (alignments) alignments 3.9 GB link
ENCFF709UGC ENCFF709UGC.bam bam (alignments) alignments 4.4 GB link
ENCFF721DJW ENCFF721DJW.bam bam (alignments) alignments 5.7 GB link
ENCFF755PSA ENCFF755PSA.bam bam (alignments) alignments 5.7 GB link
ENCFF912PRH ENCFF912PRH.bam bam (alignments) alignments 4.6 GB link
ENCFF945DFM ENCFF945DFM.bam bam (alignments) alignments 4.0 GB link
ENCFF017QBY ENCFF017QBY.bam bam (transcriptome alignments) transcriptome alignments 5.9 GB link
ENCFF128GYM ENCFF128GYM.bam bam (transcriptome alignments) transcriptome alignments 5.1 GB link
ENCFF221ADX ENCFF221ADX.bam bam (transcriptome alignments) transcriptome alignments 5.0 GB link
ENCFF259VZP ENCFF259VZP.bam bam (transcriptome alignments) transcriptome alignments 5.7 GB link
ENCFF758KRK ENCFF758KRK.bam bam (transcriptome alignments) transcriptome alignments 6.6 GB link
ENCFF994QMB ENCFF994QMB.bam bam (transcriptome alignments) transcriptome alignments 5.8 GB link
ENCFF060WZB ENCFF060WZB.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 101.3 MB link
ENCFF069ECU ENCFF069ECU.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 82.1 MB link
ENCFF175GPY ENCFF175GPY.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 79.2 MB link
ENCFF551IXO ENCFF551IXO.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 94.1 MB link
ENCFF579ILK ENCFF579ILK.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 86.4 MB link
ENCFF631ABE ENCFF631ABE.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 101.3 MB link
ENCFF773IFH ENCFF773IFH.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 90.2 MB link
ENCFF783IAV ENCFF783IAV.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 99.1 MB link
ENCFF791RRB ENCFF791RRB.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 88.2 MB link
ENCFF799IGA ENCFF799IGA.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 92.6 MB link
ENCFF877MGC ENCFF877MGC.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 94.1 MB link
ENCFF907EOW ENCFF907EOW.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 82.5 MB link
ENCFF067ZVF ENCFF067ZVF.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 82.1 MB link
ENCFF087QDS ENCFF087QDS.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 82.5 MB link
ENCFF116LEI ENCFF116LEI.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 75.8 MB link
ENCFF121QSW ENCFF121QSW.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 70.5 MB link
ENCFF369SSX ENCFF369SSX.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 82.0 MB link
ENCFF391POU ENCFF391POU.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 88.4 MB link
ENCFF420RSL ENCFF420RSL.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 88.0 MB link
ENCFF468NUA ENCFF468NUA.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 82.0 MB link
ENCFF497FNQ ENCFF497FNQ.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 81.4 MB link
ENCFF627VBI ENCFF627VBI.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 78.8 MB link
ENCFF734FKJ ENCFF734FKJ.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 88.5 MB link
ENCFF782ACV ENCFF782ACV.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 75.1 MB link
ENCFF073AWQ ENCFF073AWQ.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 100.5 MB link
ENCFF102GZP ENCFF102GZP.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 102.7 MB link
ENCFF208EHD ENCFF208EHD.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 93.8 MB link
ENCFF238CLE ENCFF238CLE.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 95.3 MB link
ENCFF488QSM ENCFF488QSM.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 102.7 MB link
ENCFF629RBE ENCFF629RBE.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 89.6 MB link
ENCFF700DVW ENCFF700DVW.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 84.1 MB link
ENCFF742NJU ENCFF742NJU.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 80.6 MB link
ENCFF745JHN ENCFF745JHN.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 83.2 MB link
ENCFF909WRQ ENCFF909WRQ.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 92.0 MB link
ENCFF954YFW ENCFF954YFW.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 88.0 MB link
ENCFF977EHR ENCFF977EHR.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 95.3 MB link
ENCFF082NUZ ENCFF082NUZ.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 90.8 MB link
ENCFF226VXY ENCFF226VXY.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 84.9 MB link
ENCFF237IRB ENCFF237IRB.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 77.6 MB link
ENCFF237LGJ ENCFF237LGJ.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 84.3 MB link
ENCFF270QUF ENCFF270QUF.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 83.5 MB link
ENCFF279VDY ENCFF279VDY.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 84.2 MB link
ENCFF458QTX ENCFF458QTX.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 81.2 MB link
ENCFF575RMG ENCFF575RMG.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 84.1 MB link
ENCFF658LZH ENCFF658LZH.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 90.3 MB link
ENCFF878BQL ENCFF878BQL.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 77.2 MB link
ENCFF937IMC ENCFF937IMC.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 90.9 MB link
ENCFF963SKW ENCFF963SKW.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 72.6 MB link
ENCFF787GPF ENCFF787GPF.fastq fastq (reads) reads 2.4 GB link
ENCFF948JBB ENCFF948JBB.fastq fastq (reads) reads 2.5 GB link
ENCFF988DMQ ENCFF988DMQ.fastq fastq (reads) reads 2.8 GB link
ENCFF990RVT ENCFF990RVT.fastq fastq (reads) reads 2.8 GB link
ENCFF309ERQ ENCFF309ERQ.tar tar (differential splicing quantifications) differential splicing quantifications 3.9 MB link
ENCFF437XYF ENCFF437XYF.tar tar (differential splicing quantifications) differential splicing quantifications 3.4 MB link
ENCFF469MJY ENCFF469MJY.tar tar (differential splicing quantifications) differential splicing quantifications 16.5 MB link
ENCFF337ZID ENCFF337ZID.tsv tsv (differential expression quantifications) differential expression quantifications 5.4 MB link
ENCFF469MYY ENCFF469MYY.tsv tsv (differential expression quantifications) differential expression quantifications 2.9 MB link
ENCFF831LVB ENCFF831LVB.tsv tsv (differential expression quantifications) differential expression quantifications 1.9 MB link
ENCFF950LTI ENCFF950LTI.tsv tsv (differential expression quantifications) differential expression quantifications 1.7 MB link
ENCFF978TSB ENCFF978TSB.tsv tsv (differential expression quantifications) differential expression quantifications 4.2 MB link
ENCFF029IFE ENCFF029IFE.tsv tsv (gene quantifications) gene quantifications 10.4 MB link
ENCFF074KQD ENCFF074KQD.tsv tsv (gene quantifications) gene quantifications 9.1 MB link
ENCFF287BYU ENCFF287BYU.tsv tsv (gene quantifications) gene quantifications 9.5 MB link
ENCFF644ESW ENCFF644ESW.tsv tsv (gene quantifications) gene quantifications 10.4 MB link
ENCFF694XSD ENCFF694XSD.tsv tsv (gene quantifications) gene quantifications 9.1 MB link
ENCFF932SQO ENCFF932SQO.tsv tsv (gene quantifications) gene quantifications 9.5 MB link
ENCFF042JFD ENCFF042JFD.tsv tsv (transcript quantifications) transcript quantifications 27.4 MB link
ENCFF200UWT ENCFF200UWT.tsv tsv (transcript quantifications) transcript quantifications 27.4 MB link
ENCFF296SOK ENCFF296SOK.tsv tsv (transcript quantifications) transcript quantifications 24.9 MB link
ENCFF334AUL ENCFF334AUL.tsv tsv (transcript quantifications) transcript quantifications 29.8 MB link
ENCFF351ALQ ENCFF351ALQ.tsv tsv (transcript quantifications) transcript quantifications 24.9 MB link
ENCFF705COG ENCFF705COG.tsv tsv (transcript quantifications) transcript quantifications 25.3 MB link
ENCFF854NGS ENCFF854NGS.tsv tsv (transcript quantifications) transcript quantifications 25.3 MB link
ENCFF959ABX ENCFF959ABX.tsv tsv (transcript quantifications) transcript quantifications 29.8 MB link