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ENCSR656DQV

ENCSR ENCODE
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shRNA knockdown against PKM2 in HepG2 cells followed by RNA-seq. (PKM2_BGHLV16)

Organism: Homo sapiens
Experiment Types:
shRNA RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

shRNA RNA-seq of PKM in Homo sapiens HepG2 expressing RNAi targeting H. sapiens PKM

Overall Design

shRNA knockdown against PKM2 in HepG2 cells followed by RNA-seq. (PKM2_BGHLV16)

Analysis page is available for ENCSR656DQV.

Dataset Citations (3)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 63.9 GB
Assay: shRNA RNA-seq
Target: PKM
Biosample: Homo sapiens HepG2 expressing RNAi targeting H. sapiens PKM
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF025FSX ENCFF025FSX.bam bam (alignments) alignments 2.5 GB link
ENCFF249PUC ENCFF249PUC.bam bam (alignments) alignments 3.0 GB link
ENCFF283TLS ENCFF283TLS.bam bam (alignments) alignments 2.8 GB link
ENCFF459GYE ENCFF459GYE.bam bam (alignments) alignments 3.0 GB link
ENCFF474EDD ENCFF474EDD.bam bam (alignments) alignments 2.8 GB link
ENCFF556NSH ENCFF556NSH.bam bam (alignments) alignments 2.4 GB link
ENCFF617SLZ ENCFF617SLZ.bam bam (alignments) alignments 2.5 GB link
ENCFF658PZD ENCFF658PZD.bam bam (alignments) alignments 2.5 GB link
ENCFF662XNN ENCFF662XNN.bam bam (alignments) alignments 3.0 GB link
ENCFF747RMM ENCFF747RMM.bam bam (alignments) alignments 2.8 GB link
ENCFF821UZD ENCFF821UZD.bam bam (alignments) alignments 2.4 GB link
ENCFF966ABL ENCFF966ABL.bam bam (alignments) alignments 2.4 GB link
ENCFF159GYM ENCFF159GYM.bam bam (transcriptome alignments) transcriptome alignments 3.9 GB link
ENCFF164FNO ENCFF164FNO.bam bam (transcriptome alignments) transcriptome alignments 3.7 GB link
ENCFF267FOO ENCFF267FOO.bam bam (transcriptome alignments) transcriptome alignments 3.5 GB link
ENCFF567TOM ENCFF567TOM.bam bam (transcriptome alignments) transcriptome alignments 3.5 GB link
ENCFF743PBZ ENCFF743PBZ.bam bam (transcriptome alignments) transcriptome alignments 4.2 GB link
ENCFF753AJO ENCFF753AJO.bam bam (transcriptome alignments) transcriptome alignments 3.7 GB link
ENCFF038AST ENCFF038AST.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 57.3 MB link
ENCFF169BRA ENCFF169BRA.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 53.8 MB link
ENCFF218NAN ENCFF218NAN.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 57.6 MB link
ENCFF273QVP ENCFF273QVP.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 49.7 MB link
ENCFF285ZYK ENCFF285ZYK.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 52.9 MB link
ENCFF413VFC ENCFF413VFC.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 52.3 MB link
ENCFF429CZL ENCFF429CZL.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 60.5 MB link
ENCFF437SFJ ENCFF437SFJ.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 59.7 MB link
ENCFF636WIY ENCFF636WIY.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 60.5 MB link
ENCFF750WPJ ENCFF750WPJ.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 51.7 MB link
ENCFF762IBZ ENCFF762IBZ.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 54.6 MB link
ENCFF881QIF ENCFF881QIF.bigWig bigWig (minus strand signal of all reads) minus strand signal of all reads 57.3 MB link
ENCFF023BEY ENCFF023BEY.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 48.5 MB link
ENCFF041BSV ENCFF041BSV.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 49.0 MB link
ENCFF079EVA ENCFF079EVA.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 54.3 MB link
ENCFF106IOF ENCFF106IOF.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 51.8 MB link
ENCFF186IEL ENCFF186IEL.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 46.7 MB link
ENCFF257AOW ENCFF257AOW.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 50.5 MB link
ENCFF310BMQ ENCFF310BMQ.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 54.0 MB link
ENCFF479LAM ENCFF479LAM.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 50.9 MB link
ENCFF506HGA ENCFF506HGA.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 49.0 MB link
ENCFF655IFV ENCFF655IFV.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 51.4 MB link
ENCFF720NTT ENCFF720NTT.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 54.4 MB link
ENCFF962DWG ENCFF962DWG.bigWig bigWig (minus strand signal of unique reads) minus strand signal of unique reads 52.1 MB link
ENCFF040UVN ENCFF040UVN.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 58.6 MB link
ENCFF094XMS ENCFF094XMS.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 61.8 MB link
ENCFF146VTO ENCFF146VTO.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 58.5 MB link
ENCFF172MJL ENCFF172MJL.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 54.0 MB link
ENCFF218RNH ENCFF218RNH.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 58.5 MB link
ENCFF307EAT ENCFF307EAT.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 61.7 MB link
ENCFF325ZGJ ENCFF325ZGJ.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 55.8 MB link
ENCFF670ZOX ENCFF670ZOX.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 53.5 MB link
ENCFF683WZW ENCFF683WZW.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 52.8 MB link
ENCFF799SIM ENCFF799SIM.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 50.8 MB link
ENCFF931TPY ENCFF931TPY.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 60.8 MB link
ENCFF989GTS ENCFF989GTS.bigWig bigWig (plus strand signal of all reads) plus strand signal of all reads 54.9 MB link
ENCFF063EOV ENCFF063EOV.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 55.6 MB link
ENCFF159YJC ENCFF159YJC.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 53.6 MB link
ENCFF332VMP ENCFF332VMP.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 55.9 MB link
ENCFF545LUG ENCFF545LUG.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 48.1 MB link
ENCFF585QJV ENCFF585QJV.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 50.0 MB link
ENCFF589VLW ENCFF589VLW.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 53.2 MB link
ENCFF615XIN ENCFF615XIN.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 52.3 MB link
ENCFF766GDR ENCFF766GDR.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 50.4 MB link
ENCFF825HOB ENCFF825HOB.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 50.3 MB link
ENCFF892AED ENCFF892AED.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 52.8 MB link
ENCFF903PDQ ENCFF903PDQ.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 51.8 MB link
ENCFF963VDL ENCFF963VDL.bigWig bigWig (plus strand signal of unique reads) plus strand signal of unique reads 55.8 MB link
ENCFF424TEQ ENCFF424TEQ.fastq fastq (reads) reads 1.7 GB link
ENCFF461GAS ENCFF461GAS.fastq fastq (reads) reads 1.5 GB link
ENCFF961CLP ENCFF961CLP.fastq fastq (reads) reads 1.6 GB link
ENCFF968UER ENCFF968UER.fastq fastq (reads) reads 1.6 GB link
ENCFF444MFG ENCFF444MFG.tar tar (differential splicing quantifications) differential splicing quantifications 13.0 MB link
ENCFF619YQQ ENCFF619YQQ.tar tar (differential splicing quantifications) differential splicing quantifications 2.7 MB link
ENCFF986IQC ENCFF986IQC.tar tar (differential splicing quantifications) differential splicing quantifications 3.0 MB link
ENCFF122TBL ENCFF122TBL.tsv tsv (differential expression quantifications) differential expression quantifications 2.8 MB link
ENCFF349XKA ENCFF349XKA.tsv tsv (differential expression quantifications) differential expression quantifications 5.5 MB link
ENCFF906UJE ENCFF906UJE.tsv tsv (differential expression quantifications) differential expression quantifications 4.1 MB link
ENCFF931QIC ENCFF931QIC.tsv tsv (differential expression quantifications) differential expression quantifications 1.9 MB link
ENCFF939XTC ENCFF939XTC.tsv tsv (differential expression quantifications) differential expression quantifications 1.8 MB link
ENCFF095EHQ ENCFF095EHQ.tsv tsv (gene quantifications) gene quantifications 9.4 MB link
ENCFF209RHL ENCFF209RHL.tsv tsv (gene quantifications) gene quantifications 9.1 MB link
ENCFF387HMK ENCFF387HMK.tsv tsv (gene quantifications) gene quantifications 9.4 MB link
ENCFF653JRQ ENCFF653JRQ.tsv tsv (gene quantifications) gene quantifications 10.4 MB link
ENCFF707XLL ENCFF707XLL.tsv tsv (gene quantifications) gene quantifications 9.1 MB link
ENCFF957MHG ENCFF957MHG.tsv tsv (gene quantifications) gene quantifications 10.4 MB link
ENCFF583XEH ENCFF583XEH.tsv tsv (transcript quantifications) transcript quantifications 29.8 MB link
ENCFF664KBN ENCFF664KBN.tsv tsv (transcript quantifications) transcript quantifications 25.3 MB link
ENCFF793RTI ENCFF793RTI.tsv tsv (transcript quantifications) transcript quantifications 24.9 MB link
ENCFF834UYZ ENCFF834UYZ.tsv tsv (transcript quantifications) transcript quantifications 27.2 MB link
ENCFF869LCR ENCFF869LCR.tsv tsv (transcript quantifications) transcript quantifications 24.9 MB link
ENCFF900FSP ENCFF900FSP.tsv tsv (transcript quantifications) transcript quantifications 25.3 MB link
ENCFF951BIX ENCFF951BIX.tsv tsv (transcript quantifications) transcript quantifications 27.2 MB link
ENCFF996AIH ENCFF996AIH.tsv tsv (transcript quantifications) transcript quantifications 29.8 MB link