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ENCSR313CHR

ENCSR ENCODE
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shRNA knockdown against EIF4B in HepG2 cells followed by RNA-seq. (EIF4B-BGHLV12)

Organism: Homo sapiens
Experiment Types:
shRNA RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

shRNA RNA-seq of EIF4B in Homo sapiens HepG2 expressing RNAi targeting H. sapiens EIF4B

Overall Design

shRNA knockdown against EIF4B in HepG2 cells followed by RNA-seq. (EIF4B-BGHLV12)

Analysis page is available for ENCSR313CHR.

Dataset Citations (3)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 123.8 GB
Assay: shRNA RNA-seq
Target: EIF4B
Biosample: Homo sapiens HepG2 expressing RNAi targeting H. sapiens EIF4B
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF273KOC ENCFF273KOC.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 4.7 GB link
ENCFF658XDT ENCFF658XDT.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 4.8 GB link
ENCFF721YQT ENCFF721YQT.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 5.7 GB link
ENCFF815NQY ENCFF815NQY.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 5.9 GB link
ENCFF280LNI ENCFF280LNI.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 5.7 GB link
ENCFF419AGX ENCFF419AGX.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 5.9 GB link
ENCFF002YLP ENCFF002YLP.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 5.6 GB link
ENCFF165UVA ENCFF165UVA.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 4.7 GB link
ENCFF210BGD ENCFF210BGD.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 4.7 GB link
ENCFF400ARN ENCFF400ARN.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 5.5 GB link
ENCFF501TAD ENCFF501TAD.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 4.7 GB link
ENCFF605FNS ENCFF605FNS.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 4.6 GB link
ENCFF140RHF ENCFF140RHF.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 7.3 GB link
ENCFF644FXA ENCFF644FXA.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 7.0 GB link
ENCFF569DOA ENCFF569DOA.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 7.8 GB link
ENCFF666PHU ENCFF666PHU.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 8.2 GB link
ENCFF228MRK ENCFF228MRK.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 7.2 GB link
ENCFF291HBJ ENCFF291HBJ.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 6.9 GB link
ENCFF106ABL ENCFF106ABL.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 96.1 MB link
ENCFF381QQH ENCFF381QQH.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 98.1 MB link
ENCFF460DLP ENCFF460DLP.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 105.2 MB link
ENCFF477QGB ENCFF477QGB.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 89.2 MB link
ENCFF034RDR ENCFF034RDR.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 105.2 MB link
ENCFF468CJV ENCFF468CJV.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 98.2 MB link
ENCFF296FJE ENCFF296FJE.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 104.8 MB link
ENCFF431BLH ENCFF431BLH.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 96.9 MB link
ENCFF504CFY ENCFF504CFY.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 91.2 MB link
ENCFF628IGB ENCFF628IGB.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 97.4 MB link
ENCFF787HGS ENCFF787HGS.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 91.1 MB link
ENCFF980VCM ENCFF980VCM.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 87.4 MB link
ENCFF079QXY ENCFF079QXY.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 88.7 MB link
ENCFF214ZPT ENCFF214ZPT.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 89.3 MB link
ENCFF933EZW ENCFF933EZW.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 94.3 MB link
ENCFF943ZOR ENCFF943ZOR.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 83.8 MB link
ENCFF583IHQ ENCFF583IHQ.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 88.6 MB link
ENCFF672ULY ENCFF672ULY.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 94.9 MB link
ENCFF027SYT ENCFF027SYT.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 84.5 MB link
ENCFF067JTT ENCFF067JTT.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 88.7 MB link
ENCFF138UAV ENCFF138UAV.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 84.9 MB link
ENCFF285IEY ENCFF285IEY.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 80.6 MB link
ENCFF670OJY ENCFF670OJY.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 89.9 MB link
ENCFF907CFI ENCFF907CFI.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 94.0 MB link
ENCFF195WFR ENCFF195WFR.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 106.7 MB link
ENCFF225SWE ENCFF225SWE.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 90.6 MB link
ENCFF660QUA ENCFF660QUA.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 99.6 MB link
ENCFF793HYY ENCFF793HYY.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 97.6 MB link
ENCFF034TBY ENCFF034TBY.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 106.7 MB link
ENCFF986BFB ENCFF986BFB.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 99.7 MB link
ENCFF088XNA ENCFF088XNA.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 106.0 MB link
ENCFF094NPQ ENCFF094NPQ.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 92.4 MB link
ENCFF284OAQ ENCFF284OAQ.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 88.9 MB link
ENCFF371HBP ENCFF371HBP.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 92.8 MB link
ENCFF454IBA ENCFF454IBA.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 98.6 MB link
ENCFF722ILX ENCFF722ILX.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 98.6 MB link
ENCFF435BVV ENCFF435BVV.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 96.5 MB link
ENCFF736ZQX ENCFF736ZQX.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 91.3 MB link
ENCFF778PLL ENCFF778PLL.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 85.7 MB link
ENCFF975GIK ENCFF975GIK.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 90.8 MB link
ENCFF811SHN ENCFF811SHN.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 90.7 MB link
ENCFF884CSN ENCFF884CSN.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 97.1 MB link
ENCFF099TFO ENCFF099TFO.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 87.0 MB link
ENCFF527IMM ENCFF527IMM.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 82.6 MB link
ENCFF659AVO ENCFF659AVO.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 86.5 MB link
ENCFF716JLO ENCFF716JLO.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 96.2 MB link
ENCFF733ZHZ ENCFF733ZHZ.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 92.1 MB link
ENCFF844QJA ENCFF844QJA.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 90.8 MB link
ENCFF113MYQ ENCFF113MYQ.fastq fastq (reads) reads 3.1 GB link
ENCFF181RDF ENCFF181RDF.fastq fastq (reads) reads 3.1 GB link
ENCFF528RFZ ENCFF528RFZ.fastq fastq (reads) reads 3.1 GB link
ENCFF577TKY ENCFF577TKY.fastq fastq (reads) reads 3.0 GB link
ENCFF572GUK ENCFF572GUK.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 3.4 MB link
ENCFF869XKA ENCFF869XKA.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 4.2 MB link
ENCFF955IBM ENCFF955IBM.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 16.5 MB link
ENCFF446HTW ENCFF446HTW.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.8 MB link
ENCFF757OSV ENCFF757OSV.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 3.0 MB link
ENCFF816SUR ENCFF816SUR.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 4.4 MB link
ENCFF877LWC ENCFF877LWC.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.9 MB link
ENCFF917PSB ENCFF917PSB.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 5.6 MB link
ENCFF222ALI ENCFF222ALI.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF968AIR ENCFF968AIR.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.4 MB link
ENCFF597CPX ENCFF597CPX.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF691TOX ENCFF691TOX.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF691ULQ ENCFF691ULQ.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF721RJD ENCFF721RJD.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF066AGE ENCFF066AGE.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF348HTQ ENCFF348HTQ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF015SRR ENCFF015SRR.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF388RHN ENCFF388RHN.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF606AVW ENCFF606AVW.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF671DFW ENCFF671DFW.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF344IQE ENCFF344IQE.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link
ENCFF849CHE ENCFF849CHE.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link