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ENCSR338CON

ENCSR ENCODE
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shRNA knockdown against AKAP1 in K562 cells followed by RNA-seq. (AKAP1-LV08)

Organism: Homo sapiens
Experiment Types:
shRNA RNA-seq
Status: released
Contact: Brenton Graveley, UConn

Summary

shRNA RNA-seq of AKAP1 in Homo sapiens K562 expressing RNAi targeting H. sapiens AKAP1

Overall Design

shRNA knockdown against AKAP1 in K562 cells followed by RNA-seq. (AKAP1-LV08)

Analysis page is available for ENCSR338CON.

Dataset Citations (3)

A large-scale binding and functional map of human RNA-binding proteins.
PMID 32728246 · 2020 · Nature
Eric L Van Nostrand, Peter Freese, Gabriel A Pratt, Xiaofeng Wang, Xintao Wei, Rui Xiao, Steven M Blue, Jia-Yu Chen, Neal A L Cody, Daniel Dominguez, Sara Olson, Balaji Sundararaman, Lijun Zhan, Cassandra Bazile, Louis Philip Benoit Bouvrette, Julie Bergalet, Michael O Duff, Keri E Garcia, Chelsea Gelboin-Burkhart, Myles Hochman, Nicole J Lambert, Hairi Li, Michael P McGurk, Thai B Nguyen, Tsultrim Palden, Ines Rabano, Shashank Sathe, Rebecca Stanton, Amanda Su, Ruth Wang, Brian A Yee, Bing Zhou, Ashley L Louie, Stefan Aigner, Xiang-Dong Fu, Eric Lécuyer, Christopher B Burge, Brenton R Graveley, Gene W Yeo

ENCODE Metadata

Total file size: 91.8 GB
Assay: shRNA RNA-seq
Target: AKAP1
Biosample: Homo sapiens K562 expressing RNAi targeting H. sapiens AKAP1
Files: 92
Unique ENC file IDs: 92
File Formats
bigWig (48) tsv (19) bam (18) fastq (4) tar (3)
Output types (11)
alignments (12) minus strand signal of all reads (12) minus strand signal of unique reads (12) plus strand signal of all reads (12) plus strand signal of unique reads (12) transcript quantifications (8) gene quantifications (6) transcriptome alignments (6) differential expression quantifications (5) reads (4) differential splicing quantifications (3)

Linked Publications (1)

Data Files (92)

Accession File Name Stored Type Output Type Mapping Assembly Size Download
ENCFF179UJS ENCFF179UJS.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.9 GB link
ENCFF275NYS ENCFF275NYS.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 4.7 GB link
ENCFF409WZH ENCFF409WZH.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 4.4 GB link
ENCFF885CJC ENCFF885CJC.bam bam (alignments); assembly: GRCh38; genome annotation: V24 alignments GRCh38 3.7 GB link
ENCFF377UTD ENCFF377UTD.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.4 GB link
ENCFF962ALJ ENCFF962ALJ.bam bam (alignments); assembly: GRCh38; genome annotation: V29 alignments GRCh38 4.7 GB link
ENCFF059NDC ENCFF059NDC.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.6 GB link
ENCFF075FRX ENCFF075FRX.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.8 GB link
ENCFF446YQT ENCFF446YQT.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.6 GB link
ENCFF527DFW ENCFF527DFW.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 4.3 GB link
ENCFF825OSD ENCFF825OSD.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 4.4 GB link
ENCFF893OHK ENCFF893OHK.bam bam (alignments); assembly: hg19; genome annotation: V19 alignments hg19 3.8 GB link
ENCFF666TAZ ENCFF666TAZ.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 4.7 GB link
ENCFF691FHB ENCFF691FHB.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V24 transcriptome alignments GRCh38 4.7 GB link
ENCFF119LCR ENCFF119LCR.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.2 GB link
ENCFF881SZI ENCFF881SZI.bam bam (transcriptome alignments); assembly: GRCh38; genome annotation: V29 transcriptome alignments GRCh38 5.2 GB link
ENCFF070VTX ENCFF070VTX.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 4.6 GB link
ENCFF678UEX ENCFF678UEX.bam bam (transcriptome alignments); assembly: hg19; genome annotation: V19 transcriptome alignments hg19 4.6 GB link
ENCFF129LXJ ENCFF129LXJ.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 82.6 MB link
ENCFF300TLA ENCFF300TLA.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 96.0 MB link
ENCFF561YSC ENCFF561YSC.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 76.8 MB link
ENCFF650YAG ENCFF650YAG.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V24 minus strand signal of all reads GRCh38 89.1 MB link
ENCFF032AMA ENCFF032AMA.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 96.1 MB link
ENCFF304MOC ENCFF304MOC.bigWig bigWig (minus strand signal of all reads); assembly: GRCh38; genome annotation: V29 minus strand signal of all reads GRCh38 89.1 MB link
ENCFF057ZCF ENCFF057ZCF.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 87.5 MB link
ENCFF435HPO ENCFF435HPO.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 79.4 MB link
ENCFF635LYD ENCFF635LYD.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 84.8 MB link
ENCFF707AAF ENCFF707AAF.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 93.8 MB link
ENCFF758MMI ENCFF758MMI.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 74.4 MB link
ENCFF965NHC ENCFF965NHC.bigWig bigWig (minus strand signal of all reads); assembly: hg19; genome annotation: V19 minus strand signal of all reads hg19 78.2 MB link
ENCFF429ABV ENCFF429ABV.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 83.2 MB link
ENCFF583LJO ENCFF583LJO.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 71.9 MB link
ENCFF789UTB ENCFF789UTB.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 77.2 MB link
ENCFF865FBH ENCFF865FBH.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 minus strand signal of unique reads GRCh38 76.4 MB link
ENCFF571AIF ENCFF571AIF.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 78.1 MB link
ENCFF936PBS ENCFF936PBS.bigWig bigWig (minus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 minus strand signal of unique reads GRCh38 84.2 MB link
ENCFF056ACD ENCFF056ACD.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 73.2 MB link
ENCFF308IDY ENCFF308IDY.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 67.9 MB link
ENCFF682FOV ENCFF682FOV.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 72.3 MB link
ENCFF723TZD ENCFF723TZD.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 76.8 MB link
ENCFF745UPO ENCFF745UPO.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 84.0 MB link
ENCFF916JSU ENCFF916JSU.bigWig bigWig (minus strand signal of unique reads); assembly: hg19; genome annotation: V19 minus strand signal of unique reads hg19 77.9 MB link
ENCFF066CIA ENCFF066CIA.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 97.8 MB link
ENCFF086LTO ENCFF086LTO.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 78.1 MB link
ENCFF773ZUP ENCFF773ZUP.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 90.5 MB link
ENCFF936HJY ENCFF936HJY.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V24 plus strand signal of all reads GRCh38 84.1 MB link
ENCFF457LGU ENCFF457LGU.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 97.8 MB link
ENCFF527VUU ENCFF527VUU.bigWig bigWig (plus strand signal of all reads); assembly: GRCh38; genome annotation: V29 plus strand signal of all reads GRCh38 90.5 MB link
ENCFF102CMX ENCFF102CMX.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 88.9 MB link
ENCFF184MZJ ENCFF184MZJ.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 86.8 MB link
ENCFF318BCU ENCFF318BCU.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 95.5 MB link
ENCFF366AMY ENCFF366AMY.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 79.9 MB link
ENCFF659NBH ENCFF659NBH.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 76.0 MB link
ENCFF771CVE ENCFF771CVE.bigWig bigWig (plus strand signal of all reads); assembly: hg19; genome annotation: V19 plus strand signal of all reads hg19 81.2 MB link
ENCFF441YKS ENCFF441YKS.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 85.7 MB link
ENCFF462SZI ENCFF462SZI.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 78.6 MB link
ENCFF590XNR ENCFF590XNR.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 79.4 MB link
ENCFF680MXI ENCFF680MXI.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V24 plus strand signal of unique reads GRCh38 73.8 MB link
ENCFF405DNR ENCFF405DNR.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 86.8 MB link
ENCFF789EGA ENCFF789EGA.bigWig bigWig (plus strand signal of unique reads); assembly: GRCh38; genome annotation: V29 plus strand signal of unique reads GRCh38 80.3 MB link
ENCFF325IPH ENCFF325IPH.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 86.8 MB link
ENCFF379QQY ENCFF379QQY.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 79.3 MB link
ENCFF554DIO ENCFF554DIO.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 74.5 MB link
ENCFF567SRK ENCFF567SRK.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 80.3 MB link
ENCFF625WOE ENCFF625WOE.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 75.6 MB link
ENCFF840LUR ENCFF840LUR.bigWig bigWig (plus strand signal of unique reads); assembly: hg19; genome annotation: V19 plus strand signal of unique reads hg19 70.1 MB link
ENCFF714OWM ENCFF714OWM.fastq fastq (reads) reads 2.4 GB link
ENCFF804VKH ENCFF804VKH.fastq fastq (reads) reads 2.2 GB link
ENCFF835GXJ ENCFF835GXJ.fastq fastq (reads) reads 2.3 GB link
ENCFF934XRW ENCFF934XRW.fastq fastq (reads) reads 2.3 GB link
ENCFF060GRQ ENCFF060GRQ.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 3.3 MB link
ENCFF078VVQ ENCFF078VVQ.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 3.7 MB link
ENCFF695DWN ENCFF695DWN.tar tar (differential splicing quantifications); assembly: hg19; genome annotation: V19 differential splicing quantifications hg19 16.1 MB link
ENCFF251MSZ ENCFF251MSZ.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 4.2 MB link
ENCFF439OOS ENCFF439OOS.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.9 MB link
ENCFF554KHL ENCFF554KHL.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 1.7 MB link
ENCFF604SAI ENCFF604SAI.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 5.4 MB link
ENCFF929PHX ENCFF929PHX.tsv tsv (differential expression quantifications); assembly: hg19; genome annotation: V19 differential expression quantifications hg19 2.9 MB link
ENCFF650HIW ENCFF650HIW.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.5 MB link
ENCFF683XJD ENCFF683XJD.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V24 gene quantifications GRCh38 9.5 MB link
ENCFF087FEN ENCFF087FEN.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF895SOI ENCFF895SOI.tsv tsv (gene quantifications); assembly: GRCh38; genome annotation: V29 gene quantifications GRCh38 10.4 MB link
ENCFF406CHS ENCFF406CHS.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF436MDF ENCFF436MDF.tsv tsv (gene quantifications); assembly: hg19; genome annotation: V19 gene quantifications hg19 9.1 MB link
ENCFF119KHX ENCFF119KHX.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF264PYJ ENCFF264PYJ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V24 transcript quantifications GRCh38 25.3 MB link
ENCFF026JDH ENCFF026JDH.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF064NGZ ENCFF064NGZ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF570PEZ ENCFF570PEZ.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 27.4 MB link
ENCFF979ZLP ENCFF979ZLP.tsv tsv (transcript quantifications); assembly: GRCh38; genome annotation: V29 transcript quantifications GRCh38 29.8 MB link
ENCFF097CPA ENCFF097CPA.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link
ENCFF696BJK ENCFF696BJK.tsv tsv (transcript quantifications); assembly: hg19; genome annotation: V19 transcript quantifications hg19 24.9 MB link