GSE286096
GSE GEOInvestigating m6A-relevant gene function in neural progenitor cells using CRISPR-Cas9 genome editing and RNA-seq
Summary
This study explores the functional role of m6A-relevant genes in neural progenitor cells (NPCs) derived from iPSCs using CRISPR-Cas9-mediated genome editing. NPCs were generated from XCL-1 iPSCs and cultured in defined conditions before establishing constitutive Cas9 expression. Synthetic guide RNAs targeting eight m6A-relevant genes (FMR1, FTO, METTL14, METTL3, WTAP, YTHDF1, YTHDF2, and YTHDF3) and non-targeting controls were introduced via lipofection. The genome editing efficiency was quantified using T7 Endonuclease I assays and sequencing. RNA-seq analysis was performed to profile transcriptomic changes associated with each knockout condition. Differential expression analysis identified significant transcriptional alterations, providing insights into m6A-regulated pathways. This work highlights the molecular impact of m6A-relevant gene disruptions in neural progenitor cells, offering a foundation for understanding their roles in neural development and disease.
Overall Design
RNA-seq profiling was conducted on neural progenitor cells (NPCs) and their CRISPR-Cas9 knockout derivatives targeting eight m6A-relevant genes (FMR1, FTO, METTL14, METTL3, WTAP, YTHDF1, YTHDF2, and YTHDF3), as well as on non-targeting (NT) controls.
Analysis
View Data ProcessingSupplementary Files (2)
GEO Samples (66)
SRA Experiments (66) and Runs (66)
Total: 168143 MBRuns (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926257 | 27585312 | 8088159445 | 2636.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926256 | 20118774 | 5997838882 | 2138.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926255 | 20676462 | 6164990829 | 2135.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926254 | 18662552 | 5591414256 | 1920.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926253 | 17829479 | 5335556638 | 1837.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926252 | 26503330 | 7913987490 | 2554.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926251 | 32632027 | 9730787315 | 3141.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926250 | 36701981 | 10986741467 | 3568.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926249 | 24715420 | 7363375913 | 2421.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926248 | 26916713 | 8032590288 | 2794.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926247 | 24158567 | 7210724905 | 2485.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926246 | 26981219 | 8031172571 | 2758.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926245 | 34676485 | 10303426429 | 3518.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926244 | 14522122 | 4132001601 | 1369.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926243 | 17608090 | 5254311044 | 1863.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926242 | 21413606 | 6405947704 | 2216.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926241 | 24414304 | 7250450219 | 2452.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926240 | 23783850 | 7102071360 | 2452.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926239 | 24225528 | 7260601582 | 2369.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926238 | 30661025 | 9187871009 | 3036.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926237 | 35948428 | 10776505821 | 3533.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926236 | 21060352 | 6285556556 | 2183.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926235 | 24023476 | 7176481439 | 2482.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926234 | 20367957 | 6066233550 | 2128.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926233 | 19622780 | 5830436035 | 1990.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926232 | 38073269 | 11388526984 | 3871.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926231 | 22996076 | 6901082259 | 2284.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926230 | 22586945 | 6767997457 | 2227.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926229 | 16522693 | 4901097635 | 1620.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926228 | 19977865 | 5943158001 | 2104.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926227 | 19988702 | 5960206981 | 2054.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926226 | 24635326 | 7287806605 | 2516.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926225 | 19466038 | 5791253946 | 1999.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926224 | 37122748 | 11083579766 | 3590.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926223 | 46871049 | 14033848791 | 4588.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926222 | 39267855 | 11730801769 | 3849.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926221 | 21965639 | 6446450939 | 2112.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926220 | 25639314 | 7667887742 | 2707.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926219 | 24986253 | 7364892909 | 2528.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926218 | 22120050 | 6599784388 | 2312.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926217 | 20523801 | 6135269598 | 2120.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926216 | 28347130 | 8423405431 | 2720.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926215 | 32744702 | 9778381080 | 3151.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926214 | 35533326 | 10571650520 | 3435.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926213 | 25521409 | 7575062961 | 2470.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926212 | 15843447 | 4732214266 | 1676.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926211 | 19565245 | 5857009170 | 2020.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926210 | 20823277 | 6240168952 | 2140.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926209 | 18296469 | 5480802597 | 1892.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926208 | 26324086 | 7835933503 | 2529.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926207 | 35844742 | 10679567891 | 3413.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926206 | 28165647 | 8367848575 | 2696.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926205 | 29266332 | 8738122153 | 2927.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926204 | 19090274 | 5701513971 | 1971.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926203 | 18521627 | 5521297481 | 1898.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926202 | 20529909 | 6151697898 | 2132.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926201 | 33367753 | 9790348685 | 3160.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926200 | 29571379 | 8836905343 | 2820.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926199 | 35888920 | 10735126788 | 3505.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926198 | 17879960 | 5331886931 | 1762.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926197 | 22296761 | 6672472529 | 2323.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926196 | 20903270 | 6186735971 | 2151.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926195 | 19968263 | 5972781391 | 2054.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926194 | 38390278 | 11485587897 | 3740.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926193 | 27310492 | 8167089922 | 2662.0 | SRA |
Runs (1)
| Run | Spots | Bases | Size (MB) | Files | Link |
|---|---|---|---|---|---|
| SRR31926192 | 25504403 | 7594172160 | 2437.0 | SRA |
Linked Publications (1)
Potentially Related Publications (1)
This accession was text-mined from the PMC full text of these publications. It may be referenced for comparison or reused from another study rather than generated for them.
Data Files (66)
| Accession | File Name | Stored Type | Output Type | Mapping Assembly | Size | Download | |
|---|---|---|---|---|---|---|---|
| — | SRR31926192_GSM8717703 | RNA-Seq | 2.4 GB | link | |||
| — | SRR31926193_GSM8717702 | RNA-Seq | 2.6 GB | link | |||
| — | SRR31926194_GSM8717701 | RNA-Seq | 3.7 GB | link | |||
| — | SRR31926195_GSM8717700 | RNA-Seq | 2.0 GB | link | |||
| — | SRR31926196_GSM8717699 | RNA-Seq | 2.1 GB | link | |||
| — | SRR31926197_GSM8717698 | RNA-Seq | 2.3 GB | link | |||
| — | SRR31926198_GSM8717697 | RNA-Seq | 1.7 GB | link | |||
| — | SRR31926199_GSM8717696 | RNA-Seq | 3.4 GB | link | |||
| — | SRR31926200_GSM8717695 | RNA-Seq | 2.8 GB | link | |||
| — | SRR31926201_GSM8717694 | RNA-Seq | 3.1 GB | link | |||
| — | SRR31926202_GSM8717693 | RNA-Seq | 2.1 GB | link | |||
| — | SRR31926203_GSM8717692 | RNA-Seq | 1.9 GB | link | |||
| — | SRR31926204_GSM8717691 | RNA-Seq | 1.9 GB | link | |||
| — | SRR31926205_GSM8717690 | RNA-Seq | 2.9 GB | link | |||
| — | SRR31926206_GSM8717689 | RNA-Seq | 2.6 GB | link | |||
| — | SRR31926207_GSM8717688 | RNA-Seq | 3.3 GB | link | |||
| — | SRR31926208_GSM8717687 | RNA-Seq | 2.5 GB | link | |||
| — | SRR31926209_GSM8717686 | RNA-Seq | 1.8 GB | link | |||
| — | SRR31926210_GSM8717685 | RNA-Seq | 2.1 GB | link | |||
| — | SRR31926211_GSM8717684 | RNA-Seq | 2.0 GB | link | |||
| — | SRR31926212_GSM8717683 | RNA-Seq | 1.6 GB | link | |||
| — | SRR31926213_GSM8717682 | RNA-Seq | 2.4 GB | link | |||
| — | SRR31926214_GSM8717681 | RNA-Seq | 3.4 GB | link | |||
| — | SRR31926215_GSM8717680 | RNA-Seq | 3.1 GB | link | |||
| — | SRR31926216_GSM8717679 | RNA-Seq | 2.7 GB | link | |||
| — | SRR31926217_GSM8717678 | RNA-Seq | 2.1 GB | link | |||
| — | SRR31926218_GSM8717677 | RNA-Seq | 2.3 GB | link | |||
| — | SRR31926219_GSM8717676 | RNA-Seq | 2.5 GB | link | |||
| — | SRR31926220_GSM8717675 | RNA-Seq | 2.6 GB | link | |||
| — | SRR31926221_GSM8717674 | RNA-Seq | 2.1 GB | link | |||
| — | SRR31926222_GSM8717673 | RNA-Seq | 3.8 GB | link | |||
| — | SRR31926223_GSM8717672 | RNA-Seq | 4.5 GB | link | |||
| — | SRR31926224_GSM8717671 | RNA-Seq | 3.5 GB | link | |||
| — | SRR31926225_GSM8717670 | RNA-Seq | 2.0 GB | link | |||
| — | SRR31926226_GSM8717669 | RNA-Seq | 2.5 GB | link | |||
| — | SRR31926227_GSM8717668 | RNA-Seq | 2.0 GB | link | |||
| — | SRR31926228_GSM8717667 | RNA-Seq | 2.1 GB | link | |||
| — | SRR31926229_GSM8717666 | RNA-Seq | 1.6 GB | link | |||
| — | SRR31926230_GSM8717665 | RNA-Seq | 2.2 GB | link | |||
| — | SRR31926231_GSM8717664 | RNA-Seq | 2.2 GB | link | |||
| — | SRR31926232_GSM8717663 | RNA-Seq | 3.8 GB | link | |||
| — | SRR31926233_GSM8717662 | RNA-Seq | 1.9 GB | link | |||
| — | SRR31926234_GSM8717661 | RNA-Seq | 2.1 GB | link | |||
| — | SRR31926235_GSM8717660 | RNA-Seq | 2.4 GB | link | |||
| — | SRR31926236_GSM8717659 | RNA-Seq | 2.1 GB | link | |||
| — | SRR31926237_GSM8717658 | RNA-Seq | 3.5 GB | link | |||
| — | SRR31926238_GSM8717657 | RNA-Seq | 3.0 GB | link | |||
| — | SRR31926239_GSM8717656 | RNA-Seq | 2.3 GB | link | |||
| — | SRR31926240_GSM8717655 | RNA-Seq | 2.4 GB | link | |||
| — | SRR31926241_GSM8717654 | RNA-Seq | 2.4 GB | link | |||
| — | SRR31926242_GSM8717653 | RNA-Seq | 2.2 GB | link | |||
| — | SRR31926243_GSM8717652 | RNA-Seq | 1.8 GB | link | |||
| — | SRR31926244_GSM8717651 | RNA-Seq | 1.3 GB | link | |||
| — | SRR31926245_GSM8717650 | RNA-Seq | 3.4 GB | link | |||
| — | SRR31926246_GSM8717649 | RNA-Seq | 2.7 GB | link | |||
| — | SRR31926247_GSM8717648 | RNA-Seq | 2.4 GB | link | |||
| — | SRR31926248_GSM8717647 | RNA-Seq | 2.7 GB | link | |||
| — | SRR31926249_GSM8717646 | RNA-Seq | 2.4 GB | link | |||
| — | SRR31926250_GSM8717645 | RNA-Seq | 3.5 GB | link | |||
| — | SRR31926251_GSM8717644 | RNA-Seq | 3.1 GB | link | |||
| — | SRR31926252_GSM8717643 | RNA-Seq | 2.5 GB | link | |||
| — | SRR31926253_GSM8717642 | RNA-Seq | 1.8 GB | link | |||
| — | SRR31926254_GSM8717641 | RNA-Seq | 1.9 GB | link | |||
| — | SRR31926255_GSM8717640 | RNA-Seq | 2.1 GB | link | |||
| — | SRR31926256_GSM8717639 | RNA-Seq | 2.1 GB | link | |||
| — | SRR31926257_GSM8717638 | RNA-Seq | 2.6 GB | link |