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3 results for “Tethered function assays”

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  1. Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion.

    Nature biotechnology · 2024 · 42(9): 1429-1441

    RNA-binding proteins (RBPs) modulate alternative splicing outcomes to determine isoform expression and cellular survival. To identify RBPs that directly drive alternative exon inclusion, we developed tethered function luciferase-based splicing reporters that provide rapid, scalable and robust readouts of exon inclusion …

    PMID 38168984 DOI ↗

  2. Large-scale tethered function assays identify factors that regulate mRNA stability and translation.

    Nature structural & molecular biology · 2020 · 27(10): 989-1000

    The molecular functions of the majority of RNA-binding proteins (RBPs) remain unclear, highlighting a major bottleneck to a full understanding of gene expression regulation. Here, we develop a plasmid resource of 690 human RBPs that we subject to luciferase-based 3'-untranslated-region …

    PMID 32807991 DOI ↗

  3. Tethered Function Assays as Tools to Elucidate the Molecular Roles of RNA-Binding Proteins.

    Advances in experimental medicine and biology · 2016 · 907: 61-88

    Dynamic regulation of RNA molecules is critical to the survival and development of cells. Messenger RNAs are transcribed in the nucleus as intron-containing pre-mRNAs and bound by RNA-binding proteins, which control their fate by regulating RNA stability, splicing, polyadenylation, translation, …

    PMID 27256382 DOI ↗

Datasets 4

GSE117293 GSE · GEO

Large-scale tethered function assays identify factors that regulate mRNA stability and translation (HEK293T_RNA-seq)

Homo sapiens · GPL20301

GSE117294 GSE · GEO

Large-scale tethered function assays identify factors that regulate mRNA stability and translation

Homo sapiens · GPL20301

GSE117291 GSE · GEO

Large-scale tethered function assays identify factors that regulate mRNA stability and translation (polysome profiling)

Homo sapiens · GPL20301

GSE117290 GSE · GEO

Large-scale tethered function assays identify factors that regulate mRNA stability and translation (eCLIP)

Homo sapiens · GPL20301

Analyses 4

GSE117290 Processing Pipeline

GSE117290 · 7 steps · OTHER · code_examples

Large-scale tethered function assays identify factors that regulate mRNA stability and translation. (2020)

GSE117291 Processing Pipeline

GSE117291 · 5 steps · RNA-Seq · code_examples

Large-scale tethered function assays identify factors that regulate mRNA stability and translation. (2020)

GSE117293 Processing Pipeline

GSE117293 · 5 steps · RNA-Seq · code_examples

Large-scale tethered function assays identify factors that regulate mRNA stability and translation. (2020)

GSE117294 Processing Pipeline

GSE117294 · 5 steps · GSE · code_examples

Large-scale tethered function assays identify factors that regulate mRNA stability and translation. (2020)