RNA-binding protein CPEB1 remodels host and viral RNA landscapes.
Abstract
Host and virus interactions occurring at the post-transcriptional level are critical for infection but remain poorly understood. Here, we performed comprehensive transcriptome-wide analyses revealing that human cytomegalovirus (HCMV) infection results in widespread alternative splicing (AS), shortening of 3' untranslated regions (3' UTRs) and lengthening of poly(A)-tails in host gene transcripts. We found that the host RNA-binding protein CPEB1 was highly induced after infection, and ectopic expression of CPEB1 in noninfected cells recapitulated infection-related post-transcriptional changes. CPEB1 was also required for poly(A)-tail lengthening of viral RNAs important for productive infection. Strikingly, depletion of CPEB1 reversed infection-related cytopathology and post-transcriptional changes, and decreased productive HCMV titers. Host RNA processing was also altered in herpes simplex virus-2 (HSV-2)-infected cells, thereby indicating that this phenomenon might be a common occurrence during herpesvirus infections. We anticipate that our work may serve as a starting point for therapeutic targeting of host RNA-binding proteins in herpesvirus infections.
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Linked Datasets (4)
Transcriptome analysis of diverse cell types infected with human cytomegalovirus [array]
Homo sapiensTranscriptome analysis of diverse cell types infected with human cytomegalovirus
Homo sapiensTranscriptome analysis of diverse cell types infected with human cytomegalovirus [RNA-Seq]
Homo sapiens26 data files
| File | Type | Size |
|---|---|---|
| EC_InfectedHCMVTB40E_48h.fastq.gz | RNA-Seq | 10.5 GB |
| EC_InfectedHCMVTB40E_48h.fastq.gz | RNA-Seq | 10.5 GB |
| EC_InfectedHCMVTB40E_96h.fastq.gz | RNA-Seq | 12.8 GB |
| EC_InfectedHCMVTB40E_96h.fastq.gz | RNA-Seq | 12.8 GB |
| EC_Mock_48h.fastq.gz | RNA-Seq | 10.1 GB |
| EC_Mock_48h.fastq.gz | RNA-Seq | 10.1 GB |
| HFF_InfectedHCMVTB40E_48h.fastq.gz | RNA-Seq | 11.7 GB |
| HFF_InfectedHCMVTB40E_48h.fastq.gz | RNA-Seq | 11.7 GB |
| HFF_InfectedHCMVTB40E_96h.fastq.gz | RNA-Seq | 11.0 GB |
| HFF_InfectedHCMVTB40E_96h.fastq.gz | RNA-Seq | 11.0 GB |
| HFF_InfectedHSV2_8h.fastq.gz | RNA-Seq | 1.3 GB |
| HFF_InfectedHSV2_8h.fastq.gz | RNA-Seq | 1.3 GB |
| HFF_Mock_48h.fastq.gz | RNA-Seq | 7.7 GB |
| HFF_Mock_48h.fastq.gz | RNA-Seq | 7.7 GB |
| HFF_MockforHSV_8h.fastq.gz | RNA-Seq | 1.6 GB |
| HFF_MockforHSV_8h.fastq.gz | RNA-Seq | 1.6 GB |
| NPC_InfectedHCMVTB40E_48h.fastq.gz | RNA-Seq | 13.6 GB |
| NPC_InfectedHCMVTB40E_48h.fastq.gz | RNA-Seq | 13.6 GB |
| NPC_InfectedHCMVTB40E_96h.fastq.gz | RNA-Seq | 12.4 GB |
| NPC_InfectedHCMVTB40E_96h.fastq.gz | RNA-Seq | 12.4 GB |
| NPC_Mock_48h.fastq.gz | RNA-Seq | 13.5 GB |
| NPC_Mock_48h.fastq.gz | RNA-Seq | 13.5 GB |
| Tcell_Control_5d.fastq.gz | RNA-Seq | 7.2 GB |
| Tcell_Control_5d.fastq.gz | RNA-Seq | 7.2 GB |
| Tcell_InfectedHIV1_5d.fastq.gz | RNA-Seq | 8.8 GB |
| Tcell_InfectedHIV1_5d.fastq.gz | RNA-Seq | 8.8 GB |
RNA binding protein CPEB1 remodels host and viral RNA landscapes [RNA-Seq]
Homo sapiens18 data files
| File | Type | Size |
|---|---|---|
| CPEB1_OE1_R1_001.fastq.gz | RNA-Seq | 5.0 GB |
| CPEB1_OE1_R1_001.fastq.gz | RNA-Seq | 5.0 GB |
| CPEB1_OE2_R1_001.fastq.gz | RNA-Seq | 2.1 GB |
| CPEB1_OE2_R1_001.fastq.gz | RNA-Seq | 2.1 GB |
| GFP_OE_R1_001.fastq.gz | RNA-Seq | 5.6 GB |
| GFP_OE_R1_001.fastq.gz | RNA-Seq | 5.6 GB |
| HCMV_R1_001.fastq.gz | RNA-Seq | 5.8 GB |
| HCMV_R1_001.fastq.gz | RNA-Seq | 5.8 GB |
| infCPEB1_R3.fastq.gz | OTHER | 122.2 MB |
| infCPEB1_R3.fastq.gz | OTHER | 122.2 MB |
| inf_R3.fastq.gz | OTHER | 96.5 MB |
| inf_R3.fastq.gz | OTHER | 96.5 MB |
| Mock_R1_001.fastq.gz | RNA-Seq | 4.8 GB |
| Mock_R1_001.fastq.gz | RNA-Seq | 4.8 GB |
| siCPEB1_R1_001.fastq.gz | RNA-Seq | 4.2 GB |
| siCPEB1_R1_001.fastq.gz | RNA-Seq | 4.2 GB |
| uninf_R3.fastq.gz | OTHER | 102.6 MB |
| uninf_R3.fastq.gz | OTHER | 102.6 MB |