Heterogeneity and clonal relationships of adaptive immune cells in ulcerative colitis revealed by single-cell analyses.
Brigid S Boland,
Zhaoren He,
Matthew S Tsai,
Jocelyn G Olvera,
Kyla D Omilusik,
Han G Duong,
Eleanor S Kim,
Abigail E Limary,
Wenhao Jin,
J Justin Milner,
Bingfei Yu,
Shefali A Patel,
Tiani L Louis,
Tiffani Tysl,
Nadia S Kurd,
Alexandra Bortnick,
Lauren K Quezada,
Jad N Kanbar,
Ara Miralles,
Danny Huylebroeck,
Mark A Valasek,
Parambir S Dulai,
Siddharth Singh,
Li-Fan Lu,
Jack D Bui,
Cornelis Murre,
William J Sandborn,
Ananda W Goldrath,
Gene W Yeo,
John T Chang
Science immunology
· 2020
· Vol. 5
(50)
Abstract
Inflammatory bowel disease (IBD) encompasses a spectrum of gastrointestinal disorders driven by dysregulated immune responses against gut microbiota. We integrated single-cell RNA and antigen receptor sequencing to elucidate key components, cellular states, and clonal relationships of the peripheral and gastrointestinal mucosal immune systems in health and ulcerative colitis (UC). UC was associated with an increase in IgG1<sup>+</sup> plasma cells in colonic tissue, increased colonic regulatory T cells characterized by elevated expression of the transcription factor ZEB2, and an enrichment of a γδ T cell subset in the peripheral blood. Moreover, we observed heterogeneity in CD8<sup>+</sup> tissue-resident memory T (T<sub>RM</sub>) cells in colonic tissue, with four transcriptionally distinct states of differentiation observed across health and disease. In the setting of UC, there was a marked shift of clonally related CD8<sup>+</sup> T<sub>RM</sub> cells toward an inflammatory state, mediated, in part, by increased expression of the T-box transcription factor Eomesodermin. Together, these results provide a detailed atlas of transcriptional changes occurring in adaptive immune cells in the context of UC and suggest a role for CD8<sup>+</sup> T<sub>RM</sub> cells in IBD.
Publication Types
["Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't"]
Keywords
MeSH Terms
["Adaptive Immunity", "Animals", "Colitis, Ulcerative", "Colon", "Humans", "Immunoglobulin G", "Intraepithelial Lymphocytes", "Male", "Memory T Cells", "Mice, Transgenic", "Single-Cell Analysis", "T-Lymphocytes, Regulatory"]
Funding
S10 OD026929
NIH HHS
(United States)
P30 CA023100
NCI NIH HHS
(United States)
P30 DK120515
NIDDK NIH HHS
(United States)
R01 AI129973
NIAID NIH HHS
(United States)
P01 AI132122
NIAID NIH HHS
(United States)
P30 NS047101
NINDS NIH HHS
(United States)
R37 AI067545
NIAID NIH HHS
(United States)
U19 MH107367
NIMH NIH HHS
(United States)
R01 AI123202
NIAID NIH HHS
(United States)
T32 DK007202
NIDDK NIH HHS
(United States)
R21 DK119724
NIDDK NIH HHS
(United States)
R01 AI100880
NIAID NIH HHS
(United States)
KL2 TR001444
NCATS NIH HHS
(United States)
I01 BX003424
BLRD VA
(United States)
Z01 AI000880
Intramural NIH HHS
(United States)
R01 AI082850
NIAID NIH HHS
(United States)
S10 OD018499
NIH HHS
(United States)
K23 DK117058
NIDDK NIH HHS
(United States)
K23 DK123406
NIDDK NIH HHS
(United States)
Linked Datasets (1)
Single-Cell Analyses Elucidate the Cellular and Molecular Landscape of Ulcerative Colitis
Homo sapiens401 data files
| File | Type | Size |
|---|---|---|
| BB06_B_S23_L002_R1_001.fastq.gz | OTHER | 3.5 GB |
| BB06_B_S23_L003_R1_001.fastq.gz | OTHER | 3.5 GB |
| BB06_T_S24_L002_R1_001.fastq.gz | OTHER | 3.7 GB |
| BB06_T_S24_L003_R1_001.fastq.gz | OTHER | 3.7 GB |
| BB07_B_S25_L002_R1_001.fastq.gz | OTHER | 4.0 GB |
| BB07_B_S25_L003_R1_001.fastq.gz | OTHER | 4.0 GB |
| BB07_T_S26_L002_R1_001.fastq.gz | OTHER | 4.2 GB |
| BB07_T_S26_L003_R1_001.fastq.gz | OTHER | 4.2 GB |
| BB08_B_S27_L002_R1_001.fastq.gz | OTHER | 3.6 GB |
| BB08_B_S27_L003_R1_001.fastq.gz | OTHER | 3.6 GB |
| BB08_T_S28_L002_R1_001.fastq.gz | OTHER | 3.5 GB |
| BB08_T_S28_L002_R2_001.fastq.gz | OTHER | 3.5 GB |
| BB08_T_S28_L003_R1_001.fastq.gz | OTHER | 3.5 GB |
| BB10_B_S31_L002_R1_001.fastq.gz | OTHER | 5.2 GB |
| BB10_B_S31_L003_R1_001.fastq.gz | OTHER | 5.2 GB |
| BB10_T_S32_L002_R1_001.fastq.gz | OTHER | 4.6 GB |
| BB10_T_S32_L002_R2_001.fastq.gz | OTHER | 4.6 GB |
| BB10_T_S32_L003_R1_001.fastq.gz | OTHER | 4.6 GB |
| BB10_T_S32_L003_R2_001.fastq.gz | OTHER | 4.6 GB |
| BB11_B_S33_L002_R1_001.fastq.gz | OTHER | 3.8 GB |
| BB11_B_S33_L003_R1_001.fastq.gz | OTHER | 3.8 GB |
| BB11_T_S34_L002_R1_001.fastq.gz | OTHER | 3.3 GB |
| BB11_T_S34_L003_R1_001.fastq.gz | OTHER | 3.3 GB |
| BB12_B_S35_L002_R1_001.fastq.gz | OTHER | 3.8 GB |
| BB12_B_S35_L003_R1_001.fastq.gz | OTHER | 3.8 GB |
| BB12_T_S36_L002_R1_001.fastq.gz | OTHER | 3.7 GB |
| BB12_T_S36_L003_R1_001.fastq.gz | OTHER | 3.8 GB |
| BB13_B_S37_L002_R1_001.fastq.gz | OTHER | 3.9 GB |
| BB13_B_S37_L003_R1_001.fastq.gz | OTHER | 3.9 GB |
| BB13_T_S38_L002_R1_001.fastq.gz | OTHER | 3.1 GB |
| BB13_T_S38_L003_R1_001.fastq.gz | OTHER | 3.1 GB |
| BB14_B_S39_L002_R1_001.fastq.gz | OTHER | 3.7 GB |
| BB14_B_S39_L003_R1_001.fastq.gz | OTHER | 3.7 GB |
| BB14_T_S40_L002_R1_001.fastq.gz | OTHER | 3.3 GB |
| BB14_T_S40_L002_R2_001.fastq.gz | OTHER | 3.3 GB |
| BB14_T_S40_L003_R1_001.fastq.gz | OTHER | 3.3 GB |
| BB14_T_S40_L003_R2_001.fastq.gz | OTHER | 3.3 GB |
| BB15_B_S41_L002_R1_001.fastq.gz | OTHER | 3.9 GB |
| BB15_B_S41_L003_R1_001.fastq.gz | OTHER | 3.9 GB |
| BB15_T_S42_L002_R1_001.fastq.gz | OTHER | 3.5 GB |
| BB15_T_S42_L003_R1_001.fastq.gz | OTHER | 3.5 GB |
| BB16_B_S43_L002_R1_001.fastq.gz | OTHER | 4.1 GB |
| BB16_B_S43_L003_R1_001.fastq.gz | OTHER | 4.1 GB |
| BB16_T_S44_L002_R1_001.fastq.gz | OTHER | 3.1 GB |
| BB16_T_S44_L003_R1_001.fastq.gz | OTHER | 3.1 GB |
| BB17_B_S45_L002_R1_001.fastq.gz | OTHER | 4.6 GB |
| BB17_B_S45_L003_R1_001.fastq.gz | OTHER | 4.6 GB |
| BB17_T_S46_L002_R1_001.fastq.gz | OTHER | 3.4 GB |
| BB17_T_S46_L003_R1_001.fastq.gz | OTHER | 3.4 GB |
| BB18_B_S47_L002_R1_001.fastq.gz | OTHER | 4.7 GB |
| BB18_B_S47_L003_R1_001.fastq.gz | OTHER | 4.6 GB |
| BB18_T_S48_L002_R1_001.fastq.gz | OTHER | 3.6 GB |
| BB18_T_S48_L003_R1_001.fastq.gz | OTHER | 3.6 GB |
| BB19_B_S49_L002_R1_001.fastq.gz | OTHER | 4.2 GB |
| BB19_B_S49_L003_R1_001.fastq.gz | OTHER | 4.2 GB |
| BB19_T_S50_L002_R1_001.fastq.gz | OTHER | 3.5 GB |
| BB19_T_S50_L003_R1_001.fastq.gz | OTHER | 3.5 GB |
| BB20_B_S51_L002_R1_001.fastq.gz | OTHER | 2.2 GB |
| BB20_B_S51_L003_R1_001.fastq.gz | OTHER | 2.2 GB |
| BB20_T_S52_L002_R1_001.fastq.gz | OTHER | 4.0 GB |
| BB20_T_S52_L003_R1_001.fastq.gz | OTHER | 4.0 GB |
| BB21_B_S53_L002_R1_001.fastq.gz | OTHER | 4.6 GB |
| BB21_B_S53_L003_R1_001.fastq.gz | OTHER | 4.6 GB |
| BB21_T_S54_L002_R1_001.fastq.gz | OTHER | 4.3 GB |
| BB21_T_S54_L003_R1_001.fastq.gz | OTHER | 4.3 GB |
| BB22_B_S55_L002_R1_001.fastq.gz | OTHER | 4.8 GB |
| BB22_B_S55_L003_R1_001.fastq.gz | OTHER | 4.8 GB |
| BB22_T_S56_L002_R1_001.fastq.gz | OTHER | 3.3 GB |
| BB22_T_S56_L002_R2_001.fastq.gz | OTHER | 3.3 GB |
| BB22_T_S56_L003_R1_001.fastq.gz | OTHER | 3.3 GB |
| BB23_T_S58_L002_R1_001.fastq.gz | OTHER | 4.4 GB |
| BB23_T_S58_L003_R1_001.fastq.gz | OTHER | 4.4 GB |
| BB24_B_S59_L002_R1_001.fastq.gz | OTHER | 3.2 GB |
| BB24_B_S59_L003_R1_001.fastq.gz | OTHER | 3.2 GB |
| BB24_T_S60_L002_R1_001.fastq.gz | OTHER | 3.8 GB |
| BB24_T_S60_L003_R1_001.fastq.gz | OTHER | 3.8 GB |
| BB25_B_S61_L002_R1_001.fastq.gz | OTHER | 4.4 GB |
| BB25_B_S61_L003_R1_001.fastq.gz | OTHER | 4.3 GB |
| BB25_T_S62_L002_R1_001.fastq.gz | OTHER | 3.7 GB |
| BB25_T_S62_L003_R1_001.fastq.gz | OTHER | 3.7 GB |
| BB28_B_S67_L002_R1_001.fastq.gz | OTHER | 3.6 GB |
| BB28_B_S67_L003_R1_001.fastq.gz | OTHER | 3.6 GB |
| BB28_T_S68_L002_R1_001.fastq.gz | OTHER | 4.8 GB |
| BB28_T_S68_L003_R1_001.fastq.gz | OTHER | 4.8 GB |
| BB29_B_S69_L002_R1_001.fastq.gz | OTHER | 4.2 GB |
| BB29_B_S69_L003_R1_001.fastq.gz | OTHER | 4.1 GB |
| BB29_T_S70_L002_R1_001.fastq.gz | OTHER | 7.9 GB |
| BB29_T_S70_L002_R2_001.fastq.gz | OTHER | 7.9 GB |
| BB29_T_S70_L003_R1_001.fastq.gz | OTHER | 7.8 GB |
| BB30_B_S71_L002_R1_001.fastq.gz | OTHER | 3.6 GB |
| BB30_B_S71_L003_R1_001.fastq.gz | OTHER | 3.6 GB |
| BB30_T_S72_L002_R1_001.fastq.gz | OTHER | 3.7 GB |
| BB30_T_S72_L003_R1_001.fastq.gz | OTHER | 3.7 GB |
| BB31_B_S73_L002_R1_001.fastq.gz | OTHER | 9.9 GB |
| BB31_B_S73_L003_R1_001.fastq.gz | OTHER | 9.9 GB |
| BB31_T_S74_L002_R1_001.fastq.gz | OTHER | 3.9 GB |
| BB31_T_S74_L003_R1_001.fastq.gz | OTHER | 3.9 GB |
| BB32_B_S75_L002_R1_001.fastq.gz | OTHER | 4.6 GB |
| BB32_B_S75_L003_R1_001.fastq.gz | OTHER | 4.5 GB |
| BB32_T_S76_L002_R1_001.fastq.gz | OTHER | 3.1 GB |
| BB32_T_S76_L003_R1_001.fastq.gz | OTHER | 3.1 GB |
| BB33_B_S77_L002_R1_001.fastq.gz | OTHER | 5.6 GB |
| BB33_B_S77_L003_R1_001.fastq.gz | OTHER | 5.6 GB |
| BB33_T_S78_L002_R1_001.fastq.gz | OTHER | 3.9 GB |
| BB33_T_S78_L003_R1_001.fastq.gz | OTHER | 3.9 GB |
| C12pBMCBCR_S13_L001_R1_001.fastq.gz | OTHER | 2.0 MB |
| C12pBMCBCR_S14_L001_R1_001.fastq.gz | OTHER | 3.0 MB |
| C12pBMCBCR_S15_L001_R1_001.fastq.gz | OTHER | 2.7 MB |
| C12pBMCBCR_S16_L001_R1_001.fastq.gz | OTHER | 1.6 MB |
| C12_pBMC_BCR_S34_L003_R1_001.fastq.gz | OTHER | 18.6 GB |
| C12pBMCSC_S65_L007_R1_001.fastq.gz | RNA-Seq | 509.4 MB |
| C12pBMCSC_S66_L007_R1_001.fastq.gz | RNA-Seq | 418.0 MB |
| C12pBMCSC_S67_L007_R1_001.fastq.gz | RNA-Seq | 422.3 MB |
| C12pBMCSC_S68_L007_R1_001.fastq.gz | RNA-Seq | 538.3 MB |
| C12pBMCTCR_S29_L001_R1_001.fastq.gz | OTHER | 17.9 MB |
| C12pBMCTCR_S30_L001_R1_001.fastq.gz | OTHER | 16.1 MB |
| C12pBMCTCR_S31_L001_R1_001.fastq.gz | OTHER | 20.6 MB |
| C12pBMCTCR_S32_L001_R1_001.fastq.gz | OTHER | 11.6 MB |
| C12_pBMC_TCR_S35_L003_R1_001.fastq.gz | OTHER | 6.3 GB |
| C12RectumBCR_S10_L001_R1_001.fastq.gz | OTHER | 48.4 MB |
| C12RectumBCR_S11_L001_R1_001.fastq.gz | OTHER | 35.3 MB |
| C12RectumBCR_S12_L001_R1_001.fastq.gz | OTHER | 50.0 MB |
| C12_Rectum_BCR_S36_L003_R1_001.fastq.gz | OTHER | 48.6 GB |
| C12RectumBCR_S9_L001_R1_001.fastq.gz | OTHER | 60.9 MB |
| C12RectumSC_S61_L007_R1_001.fastq.gz | RNA-Seq | 550.1 MB |
| C12RectumSC_S62_L007_R1_001.fastq.gz | RNA-Seq | 474.5 MB |
| C12RectumSC_S63_L007_R1_001.fastq.gz | RNA-Seq | 462.3 MB |
| C12RectumSC_S64_L007_R1_001.fastq.gz | RNA-Seq | 463.2 MB |
| C12RectumTCR_1-10_S25_L001_R1_001.fastq.gz | OTHER | 14.1 MB |
| C12RectumTCR_1-10_S26_L001_R1_001.fastq.gz | OTHER | 8.9 MB |
| C12RectumTCR_1-10_S27_L001_R1_001.fastq.gz | OTHER | 10.0 MB |
| C12RectumTCR_1-10_S28_L001_R1_001.fastq.gz | OTHER | 8.4 MB |
| C12_Rectum_TCR_S37_L003_R1_001.fastq.gz | OTHER | 12.1 GB |
| C16_pbmc_control_16_pbmc_S13_L004_R1_001.fastq.gz | RNA-Seq | 1.6 GB |
| C16_pbmc_control_16_pbmc_S14_L004_R1_001.fastq.gz | RNA-Seq | 1.5 GB |
| C16_pbmc_control_16_pbmc_S15_L004_R1_001.fastq.gz | RNA-Seq | 1.4 GB |
| C16_pbmc_control_16_pbmc_S16_L004_R1_001.fastq.gz | RNA-Seq | 1.4 GB |
| C16_P_control_16_pbmc_S1_L003_R1_001.fastq.gz | RNA-Seq | 838.1 MB |
| C16_P_control_16_pbmc_S2_L003_R1_001.fastq.gz | RNA-Seq | 789.2 MB |
| C16_P_control_16_pbmc_S3_L003_R1_001.fastq.gz | RNA-Seq | 711.0 MB |
| C16_P_control_16_pbmc_S4_L003_R1_001.fastq.gz | RNA-Seq | 717.7 MB |
| C16_R_control_16_rectum_S5_L003_R1_001.fastq.gz | RNA-Seq | 1.1 GB |
| C16_R_control_16_rectum_S6_L003_R1_001.fastq.gz | RNA-Seq | 393.8 MB |
| C16_R_control_16_rectum_S7_L003_R1_001.fastq.gz | RNA-Seq | 573.4 MB |
| C16_R_control_16_rectum_S8_L003_R1_001.fastq.gz | RNA-Seq | 579.8 MB |
| C17_I_10x_S1_L001_R1_001.fastq.gz | RNA-Seq | 128.0 MB |
| C17_I_10x_S1_L004_R1_001.fastq.gz | RNA-Seq | 192.3 MB |
| C17_I_10x_S1_L005_R1_001.fastq.gz | RNA-Seq | 188.1 MB |
| C17_I_10x_S1_L006_R1_001.fastq.gz | RNA-Seq | 189.0 MB |
| C17_I_10x_S2_L001_R1_001.fastq.gz | RNA-Seq | 117.2 MB |
| C17_I_10x_S2_L004_R1_001.fastq.gz | RNA-Seq | 166.6 MB |
| C17_I_10x_S2_L005_R1_001.fastq.gz | RNA-Seq | 162.5 MB |
| C17_I_10x_S2_L006_R1_001.fastq.gz | RNA-Seq | 164.0 MB |
| C17_I_10x_S3_L001_R1_001.fastq.gz | RNA-Seq | 253.0 MB |
| C17_I_10x_S3_L004_R1_001.fastq.gz | RNA-Seq | 361.1 MB |
| C17_I_10x_S3_L005_R1_001.fastq.gz | RNA-Seq | 351.6 MB |
| C17_I_10x_S3_L006_R1_001.fastq.gz | RNA-Seq | 356.0 MB |
| C17_I_10x_S4_L001_R1_001.fastq.gz | RNA-Seq | 119.9 MB |
| C17_I_10x_S4_L004_R1_001.fastq.gz | RNA-Seq | 169.2 MB |
| C17_I_10x_S4_L004_R1_001.fastq.gz | RNA-Seq | 169.2 MB |
| C17_I_10x_S4_L005_R1_001.fastq.gz | RNA-Seq | 165.4 MB |
| C17_I_10x_S4_L006_R1_001.fastq.gz | RNA-Seq | 167.0 MB |
| C17_I_BCR_S10_L003_R1_001.fastq.gz | OTHER | 4.3 GB |
| C17_I_TCR_S25_L003_R1_001.fastq.gz | OTHER | 5.8 GB |
| C17_pBMC_10x_S10_L001_R1_001.fastq.gz | RNA-Seq | 498.7 MB |
| C17_pBMC_10x_S10_L004_R1_001.fastq.gz | RNA-Seq | 725.6 MB |
| C17_pBMC_10x_S10_L005_R1_001.fastq.gz | RNA-Seq | 709.5 MB |
| C17_pBMC_10x_S10_L006_R1_001.fastq.gz | RNA-Seq | 712.4 MB |
| C17_pBMC_10x_S11_L001_R1_001.fastq.gz | RNA-Seq | 517.0 MB |
| C17_pBMC_10x_S11_L004_R1_001.fastq.gz | RNA-Seq | 748.7 MB |
| C17_pBMC_10x_S11_L005_R1_001.fastq.gz | RNA-Seq | 733.8 MB |
| C17_pBMC_10x_S11_L006_R1_001.fastq.gz | RNA-Seq | 739.6 MB |
| C17_pBMC_10x_S12_L001_R1_001.fastq.gz | RNA-Seq | 545.7 MB |
| C17_pBMC_10x_S12_L004_R1_001.fastq.gz | RNA-Seq | 783.0 MB |
| C17_pBMC_10x_S12_L005_R1_001.fastq.gz | RNA-Seq | 766.3 MB |
| C17_pBMC_10x_S12_L006_R1_001.fastq.gz | RNA-Seq | 772.0 MB |
| C17_pBMC_10x_S9_L001_R1_001.fastq.gz | RNA-Seq | 349.1 MB |
| C17_pBMC_10x_S9_L004_R1_001.fastq.gz | RNA-Seq | 502.4 MB |
| C17_pBMC_10x_S9_L005_R1_001.fastq.gz | RNA-Seq | 492.4 MB |
| C17_pBMC_10x_S9_L006_R1_001.fastq.gz | RNA-Seq | 496.3 MB |
| C17_pBMC_BCR_S12_L003_R1_001.fastq.gz | OTHER | 4.7 GB |
| C17_pBMC_TCR_S27_L003_R1_001.fastq.gz | OTHER | 4.6 GB |
| C17_R_10x_S5_L001_R1_001.fastq.gz | RNA-Seq | 259.5 MB |
| C17_R_10x_S5_L004_R1_001.fastq.gz | RNA-Seq | 376.3 MB |
| C17_R_10x_S5_L005_R1_001.fastq.gz | RNA-Seq | 367.3 MB |
| C17_R_10x_S5_L006_R1_001.fastq.gz | RNA-Seq | 371.2 MB |
| C17_R_10x_S6_L001_R1_001.fastq.gz | RNA-Seq | 38.9 MB |
| C17_R_10x_S6_L004_R1_001.fastq.gz | RNA-Seq | 54.7 MB |
| C17_R_10x_S6_L005_R1_001.fastq.gz | RNA-Seq | 53.3 MB |
| C17_R_10x_S6_L006_R1_001.fastq.gz | RNA-Seq | 53.4 MB |
| C17_R_10x_S7_L001_R1_001.fastq.gz | RNA-Seq | 247.4 MB |
| C17_R_10x_S7_L004_R1_001.fastq.gz | RNA-Seq | 371.9 MB |
| C17_R_10x_S7_L005_R1_001.fastq.gz | RNA-Seq | 363.3 MB |
| C17_R_10x_S7_L006_R1_001.fastq.gz | RNA-Seq | 366.2 MB |
| C17_R_10x_S8_L001_R1_001.fastq.gz | RNA-Seq | 163.9 MB |
| C17_R_10x_S8_L004_R1_001.fastq.gz | RNA-Seq | 231.2 MB |
| C17_R_10x_S8_L005_R1_001.fastq.gz | RNA-Seq | 225.6 MB |
| C17_R_10x_S8_L006_R1_001.fastq.gz | RNA-Seq | 228.5 MB |
| C17_R_BCR_S11_L003_R1_001.fastq.gz | OTHER | 2.1 GB |
| C17_R_TCR_S26_L003_R1_001.fastq.gz | OTHER | 7.8 GB |
| C18_I_10x_S13_L001_R1_001.fastq.gz | RNA-Seq | 208.1 MB |
| C18_I_10x_S13_L004_R1_001.fastq.gz | RNA-Seq | 303.3 MB |
| C18_I_10x_S13_L005_R1_001.fastq.gz | RNA-Seq | 296.1 MB |
| C18_I_10x_S13_L006_R1_001.fastq.gz | RNA-Seq | 299.7 MB |
| C18_I_10x_S14_L001_R1_001.fastq.gz | RNA-Seq | 287.1 MB |
| C18_I_10x_S14_L004_R1_001.fastq.gz | RNA-Seq | 419.6 MB |
| C18_I_10x_S14_L005_R1_001.fastq.gz | RNA-Seq | 408.9 MB |
| C18_I_10x_S14_L006_R1_001.fastq.gz | RNA-Seq | 412.3 MB |
| C18_I_10x_S15_L001_R1_001.fastq.gz | RNA-Seq | 192.9 MB |
| C18_I_10x_S15_L004_R1_001.fastq.gz | RNA-Seq | 272.8 MB |
| C18_I_10x_S15_L005_R1_001.fastq.gz | RNA-Seq | 266.3 MB |
| C18_I_10x_S15_L006_R1_001.fastq.gz | RNA-Seq | 269.2 MB |
| C18_I_10x_S16_L001_R1_001.fastq.gz | RNA-Seq | 241.9 MB |
| C18_I_10x_S16_L004_R1_001.fastq.gz | RNA-Seq | 368.2 MB |
| C18_I_10x_S16_L005_R1_001.fastq.gz | RNA-Seq | 359.7 MB |
| C18_I_10x_S16_L006_R1_001.fastq.gz | RNA-Seq | 364.1 MB |
| C18_I_BCR_S13_L003_R1_001.fastq.gz | OTHER | 8.9 GB |
| C18_I_TCR_S17_L003_R1_001.fastq.gz | OTHER | 4.6 GB |
| C18_pBMC_10x_S21_L001_R1_001.fastq.gz | RNA-Seq | 112.4 MB |
| C18_pBMC_10x_S21_L004_R1_001.fastq.gz | RNA-Seq | 166.2 MB |
| C18_pBMC_10x_S21_L005_R1_001.fastq.gz | RNA-Seq | 162.2 MB |
| C18_pBMC_10x_S21_L006_R1_001.fastq.gz | RNA-Seq | 163.1 MB |
| C18_pBMC_10x_S22_L001_R1_001.fastq.gz | RNA-Seq | 103.3 MB |
| C18_pBMC_10x_S22_L004_R1_001.fastq.gz | RNA-Seq | 149.7 MB |
| C18_pBMC_10x_S22_L005_R1_001.fastq.gz | RNA-Seq | 146.2 MB |
| C18_pBMC_10x_S22_L006_R1_001.fastq.gz | RNA-Seq | 147.6 MB |
| C18_pBMC_10x_S23_L001_R1_001.fastq.gz | RNA-Seq | 101.4 MB |
| C18_pBMC_10x_S23_L004_R1_001.fastq.gz | RNA-Seq | 142.3 MB |
| C18_pBMC_10x_S23_L005_R1_001.fastq.gz | RNA-Seq | 138.8 MB |
| C18_pBMC_10x_S23_L006_R1_001.fastq.gz | RNA-Seq | 140.3 MB |
| C18_pBMC_10x_S24_L001_R1_001.fastq.gz | RNA-Seq | 97.2 MB |
| C18_pBMC_10x_S24_L004_R1_001.fastq.gz | RNA-Seq | 139.4 MB |
| C18_pBMC_10x_S24_L005_R1_001.fastq.gz | RNA-Seq | 136.0 MB |
| C18_pBMC_10x_S24_L006_R1_001.fastq.gz | RNA-Seq | 137.6 MB |
| C18_pBMC_BCR_S15_L003_R1_001.fastq.gz | OTHER | 8.7 GB |
| C18_pBMC_TCR_S19_L003_R1_001.fastq.gz | OTHER | 5.2 GB |
| C18_R_10x_S17_L001_R1_001.fastq.gz | RNA-Seq | 138.9 MB |
| C18_R_10x_S17_L004_R1_001.fastq.gz | RNA-Seq | 200.8 MB |
| C18_R_10x_S17_L005_R1_001.fastq.gz | RNA-Seq | 195.9 MB |
| C18_R_10x_S17_L006_R1_001.fastq.gz | RNA-Seq | 198.0 MB |
| C18_R_10x_S18_L001_R1_001.fastq.gz | RNA-Seq | 972.8 KB |
| C18_R_10x_S18_L004_R1_001.fastq.gz | RNA-Seq | 1.4 MB |
| C18_R_10x_S18_L005_R1_001.fastq.gz | RNA-Seq | 1.3 MB |
| C18_R_10x_S18_L006_R1_001.fastq.gz | RNA-Seq | 1.3 MB |
| C18_R_10x_S19_L001_R1_001.fastq.gz | RNA-Seq | 115.4 MB |
| C18_R_10x_S19_L004_R1_001.fastq.gz | RNA-Seq | 168.9 MB |
| C18_R_10x_S19_L005_R1_001.fastq.gz | RNA-Seq | 165.5 MB |
| C18_R_10x_S19_L006_R1_001.fastq.gz | RNA-Seq | 166.3 MB |
| C18_R_10x_S20_L001_R1_001.fastq.gz | RNA-Seq | 168.3 MB |
| C18_R_10x_S20_L004_R1_001.fastq.gz | RNA-Seq | 249.4 MB |
| C18_R_10x_S20_L005_R1_001.fastq.gz | RNA-Seq | 244.2 MB |
| C18_R_10x_S20_L006_R1_001.fastq.gz | RNA-Seq | 246.1 MB |
| C18_R_BCR_S14_L003_R1_001.fastq.gz | OTHER | 4.4 GB |
| C18_R_TCR_S18_L003_R1_001.fastq.gz | OTHER | 4.6 GB |
| C19_ileum_Control_19_ileum_S21_L005_R1_001.fastq.gz | RNA-Seq | 919.6 MB |
| C19_ileum_Control_19_ileum_S22_L005_R1_001.fastq.gz | RNA-Seq | 1.0 GB |
| C19_ileum_Control_19_ileum_S23_L005_R1_001.fastq.gz | RNA-Seq | 691.2 MB |
| C19_ileum_Control_19_ileum_S24_L005_R1_001.fastq.gz | RNA-Seq | 1.3 GB |
| C19_ileum_S5_L005_R1_001.fastq.gz | RNA-Seq | 561.9 MB |
| C19_ileum_S6_L005_R1_001.fastq.gz | RNA-Seq | 624.6 MB |
| C19_ileum_S7_L005_R1_001.fastq.gz | RNA-Seq | 421.7 MB |
| C19_ileum_S8_L005_R1_001.fastq.gz | RNA-Seq | 778.2 MB |
| C19_pbmc_control_19_pBMC_S25_L005_R1_001.fastq.gz | RNA-Seq | 1.0 GB |
| C19_pbmc_control_19_pBMC_S26_L005_R1_001.fastq.gz | RNA-Seq | 748.0 MB |
| C19_pbmc_control_19_pBMC_S27_L005_R1_001.fastq.gz | RNA-Seq | 566.6 MB |
| C19_pbmc_control_19_pBMC_S28_L005_R1_001.fastq.gz | RNA-Seq | 650.1 MB |
| C19_pBMC_S1_L005_R1_001.fastq.gz | RNA-Seq | 815.4 MB |
| C19_pBMC_S2_L005_R1_001.fastq.gz | RNA-Seq | 585.3 MB |
| C19_pBMC_S3_L005_R1_001.fastq.gz | RNA-Seq | 447.8 MB |
| C19_pBMC_S4_L005_R1_001.fastq.gz | RNA-Seq | 512.6 MB |
| C19_rectum_control_19_rectum_S29_L005_R1_001.fastq.gz | RNA-Seq | 1.7 GB |
| C19_rectum_control_19_rectum_S30_L005_R1_001.fastq.gz | RNA-Seq | 961.8 MB |
| C19_rectum_control_19_rectum_S31_L005_R1_001.fastq.gz | RNA-Seq | 1016.0 MB |
| C19_rectum_control_19_rectum_S32_L005_R1_001.fastq.gz | RNA-Seq | 1.4 GB |
| C19_rectum_S10_L005_R1_001.fastq.gz | RNA-Seq | 446.2 MB |
| C19_rectum_S11_L005_R1_001.fastq.gz | RNA-Seq | 468.9 MB |
| C19_rectum_S12_L005_R1_001.fastq.gz | RNA-Seq | 661.1 MB |
| C19_rectum_S9_L005_R1_001.fastq.gz | RNA-Seq | 811.5 MB |
| C21_ileum_Control_21_ileum_S17_L005_R1_001.fastq.gz | RNA-Seq | 974.7 MB |
| C21_ileum_Control_21_ileum_S18_L005_R1_001.fastq.gz | RNA-Seq | 562.9 MB |
| C21_ileum_Control_21_ileum_S19_L005_R1_001.fastq.gz | RNA-Seq | 700.6 MB |
| C21_ileum_Control_21_ileum_S20_L005_R1_001.fastq.gz | RNA-Seq | 766.1 MB |
| C21_ileum_S29_L006_R1_001.fastq.gz | RNA-Seq | 754.5 MB |
| C21_ileum_S30_L006_R1_001.fastq.gz | RNA-Seq | 440.4 MB |
| C21_ileum_S31_L006_R1_001.fastq.gz | RNA-Seq | 546.6 MB |
| C21_ileum_S32_L006_R1_001.fastq.gz | RNA-Seq | 598.0 MB |
| C21_pBMC_S25_L005_R1_001.fastq.gz | RNA-Seq | 588.6 MB |
| C21_pBMC_S26_L005_R1_001.fastq.gz | RNA-Seq | 587.7 MB |
| C21_pBMC_S27_L005_R1_001.fastq.gz | RNA-Seq | 536.8 MB |
| C21_pBMC_S28_L005_R1_001.fastq.gz | RNA-Seq | 762.3 MB |
| C21_rectum_S33_L006_R1_001.fastq.gz | RNA-Seq | 932.3 MB |
| C21_rectum_S34_L006_R1_001.fastq.gz | RNA-Seq | 464.2 MB |
| C21_rectum_S35_L006_R1_001.fastq.gz | RNA-Seq | 686.2 MB |
| C21_rectum_S36_L006_R1_001.fastq.gz | RNA-Seq | 494.8 MB |
| C30_ileum_control_30_ileum_S33_L005_R1_001.fastq.gz | RNA-Seq | 707.7 MB |
| C30_ileum_control_30_ileum_S34_L005_R1_001.fastq.gz | RNA-Seq | 1.0 GB |
| C30_ileum_control_30_ileum_S35_L005_R1_001.fastq.gz | RNA-Seq | 649.5 MB |
| C30_ileum_control_30_ileum_S36_L005_R1_001.fastq.gz | RNA-Seq | 658.9 MB |
| C30_ileum_S41_L006_R1_001.fastq.gz | RNA-Seq | 599.3 MB |
| C30_ileum_S42_L006_R1_001.fastq.gz | RNA-Seq | 864.9 MB |
| C30_ileum_S43_L006_R1_001.fastq.gz | RNA-Seq | 549.8 MB |
| C30_ileum_S44_L006_R1_001.fastq.gz | RNA-Seq | 556.4 MB |
| C30_pbmc_S37_L006_R1_001.fastq.gz | RNA-Seq | 649.7 MB |
| C30_pbmc_S38_L006_R1_001.fastq.gz | RNA-Seq | 419.6 MB |
| C30_pbmc_S39_L006_R1_001.fastq.gz | RNA-Seq | 632.7 MB |
| C30_pbmc_S40_L006_R1_001.fastq.gz | RNA-Seq | 770.1 MB |
| C30_rectum_control_30_rectum_S1_L004_R1_001.fastq.gz | RNA-Seq | 1.3 GB |
| C30_rectum_control_30_rectum_S2_L004_R1_001.fastq.gz | RNA-Seq | 1.4 GB |
| C30_rectum_control_30_rectum_S3_L004_R1_001.fastq.gz | RNA-Seq | 1.8 GB |
| C30_rectum_control_30_rectum_S4_L004_R1_001.fastq.gz | RNA-Seq | 2.3 GB |
| C30_rectum_S45_L006_R1_001.fastq.gz | RNA-Seq | 481.8 MB |
| C30_rectum_S46_L006_R1_001.fastq.gz | RNA-Seq | 499.1 MB |
| C30_rectum_S47_L006_R1_001.fastq.gz | RNA-Seq | 647.3 MB |
| C30_rectum_S48_L006_R1_001.fastq.gz | RNA-Seq | 826.5 MB |
| C33_ileum_S17_L005_R1_001.fastq.gz | RNA-Seq | 728.0 MB |
| C33_ileum_S18_L005_R1_001.fastq.gz | RNA-Seq | 715.2 MB |
| C33_ileum_S19_L005_R1_001.fastq.gz | RNA-Seq | 809.3 MB |
| C33_ileum_S20_L005_R1_001.fastq.gz | RNA-Seq | 881.4 MB |
| C33_pBMC_S13_L005_R1_001.fastq.gz | RNA-Seq | 830.5 MB |
| C33_pBMC_S14_L005_R1_001.fastq.gz | RNA-Seq | 310.2 MB |
| C33_pBMC_S15_L005_R1_001.fastq.gz | RNA-Seq | 814.1 MB |
| C33_pBMC_S16_L005_R1_001.fastq.gz | RNA-Seq | 496.9 MB |
| C9RectumBCR_S1_L001_R1_001.fastq.gz | OTHER | 36.5 MB |
| C9_Rectum_BCR_S1_L003_R1_001.fastq.gz | OTHER | 4.3 GB |
| C9RectumBCR_S2_L001_R1_001.fastq.gz | OTHER | 30.7 MB |
| C9RectumBCR_S3_L001_R1_001.fastq.gz | OTHER | 36.8 MB |
| C9RectumBCR_S4_L001_R1_001.fastq.gz | OTHER | 33.1 MB |
| C9RectumSC_S53_L007_R1_001.fastq.gz | RNA-Seq | 1.2 GB |
| C9RectumSC_S54_L007_R1_001.fastq.gz | RNA-Seq | 1.3 GB |
| C9RectumSC_S55_L007_R1_001.fastq.gz | RNA-Seq | 1.3 GB |
| C9RectumSC_S56_L007_R1_001.fastq.gz | RNA-Seq | 1.6 GB |
| C9RectumTCR_S17_L001_R1_001.fastq.gz | OTHER | 99.6 MB |
| C9RectumTCR_S18_L001_R1_001.fastq.gz | OTHER | 91.5 MB |
| C9RectumTCR_S19_L001_R1_001.fastq.gz | OTHER | 100.9 MB |
| C9RectumTCR_S20_L001_R1_001.fastq.gz | OTHER | 81.1 MB |
| C9_Rectum_TCR_S2_L003_R1_001.fastq.gz | OTHER | 17.0 MB |
| SRR8478779 | OTHER | 4.2 GB |
| U34_pbmc_S49_L006_R1_001.fastq.gz | RNA-Seq | 683.5 MB |
| U34_pbmc_S50_L006_R1_001.fastq.gz | RNA-Seq | 325.7 MB |
| U34_pbmc_S51_L006_R1_001.fastq.gz | RNA-Seq | 1019.5 MB |
| U34_pbmc_S52_L006_R1_001.fastq.gz | RNA-Seq | 616.4 MB |
| U34_pBMC_ulcerative_colitis_34_pBMC_S10_L004_R1_001.fastq.gz | RNA-Seq | 301.4 MB |
| U34_pBMC_ulcerative_colitis_34_pBMC_S11_L004_R1_001.fastq.gz | RNA-Seq | 937.5 MB |
| U34_pBMC_ulcerative_colitis_34_pBMC_S12_L004_R1_001.fastq.gz | RNA-Seq | 560.9 MB |
| U34_pBMC_ulcerative_colitis_34_pBMC_S9_L004_R1_001.fastq.gz | RNA-Seq | 622.2 MB |
| U34_rectum_S53_L006_R1_001.fastq.gz | RNA-Seq | 701.6 MB |
| U34_rectum_S54_L006_R1_001.fastq.gz | RNA-Seq | 565.7 MB |
| U34_rectum_S55_L006_R1_001.fastq.gz | RNA-Seq | 709.6 MB |
| U34_rectum_S56_L006_R1_001.fastq.gz | RNA-Seq | 780.5 MB |
| U34_Rectum_Ulcerative_colitis_34_Rectum_S5_L004_R1_001.fast… | RNA-Seq | 524.8 MB |
| U34_Rectum_Ulcerative_colitis_34_Rectum_S6_L004_R1_001.fast… | RNA-Seq | 419.6 MB |
| U34_Rectum_Ulcerative_colitis_34_Rectum_S7_L004_R1_001.fast… | RNA-Seq | 524.2 MB |
| U34_Rectum_Ulcerative_colitis_34_Rectum_S8_L004_R1_001.fast… | RNA-Seq | 581.7 MB |
| U35_P_Ulcerative_colitis_35_pbmc_S1_L001_R1_001.fastq.gz | RNA-Seq | 528.6 MB |
| U35_P_Ulcerative_colitis_35_pbmc_S2_L001_R1_001.fastq.gz | RNA-Seq | 352.0 MB |
| U35_P_Ulcerative_colitis_35_pbmc_S3_L001_R1_001.fastq.gz | RNA-Seq | 864.5 MB |
| U35_P_Ulcerative_colitis_35_pbmc_S4_L001_R1_001.fastq.gz | RNA-Seq | 835.1 MB |
| U35_R_Ulcerative_colitis_35_rectum_S5_L001_R1_001.fastq.gz | RNA-Seq | 477.9 MB |
| U35_R_Ulcerative_colitis_35_rectum_S6_L001_R1_001.fastq.gz | RNA-Seq | 557.5 MB |
| U35_R_Ulcerative_colitis_35_rectum_S7_L001_R1_001.fastq.gz | RNA-Seq | 902.4 MB |
| U35_R_Ulcerative_colitis_35_rectum_S8_L001_R1_001.fastq.gz | RNA-Seq | 897.6 MB |
| U41_P_Ulcerative_colitis_41_pbmc_S10_L001_R1_001.fastq.gz | RNA-Seq | 9.8 MB |
| U41_P_Ulcerative_colitis_41_pbmc_S11_L001_R1_001.fastq.gz | RNA-Seq | 362.6 MB |
| U41_P_Ulcerative_colitis_41_pbmc_S12_L001_R1_001.fastq.gz | RNA-Seq | 495.9 MB |
| U41_P_Ulcerative_colitis_41_pbmc_S9_L001_R1_001.fastq.gz | RNA-Seq | 546.2 MB |
| U41_R_Ulcerative_colitis_41_rectum_S13_L001_R1_001.fastq.gz | RNA-Seq | 836.3 MB |
| U41_R_Ulcerative_colitis_41_rectum_S14_L001_R1_001.fastq.gz | RNA-Seq | 483.1 MB |
| U41_R_Ulcerative_colitis_41_rectum_S15_L001_R1_001.fastq.gz | RNA-Seq | 711.4 MB |
| U41_R_Ulcerative_colitis_41_rectum_S16_L001_R1_001.fastq.gz | RNA-Seq | 1007.8 MB |
| U44_pbmc_ulcerative_colitis_44_pbmc_S17_L001_R1_001.fastq.gz | RNA-Seq | 809.1 MB |
| U44_pbmc_ulcerative_colitis_44_pbmc_S18_L001_R1_001.fastq.gz | RNA-Seq | 912.8 MB |
| U44_pbmc_ulcerative_colitis_44_pbmc_S19_L001_R1_001.fastq.gz | RNA-Seq | 150.2 MB |
| U44_pbmc_ulcerative_colitis_44_pbmc_S20_L001_R1_001.fastq.gz | RNA-Seq | 738.4 MB |
| U44_rectum_ulcerative_coliis_44_rectm_S21_L001_R1_001.fastq… | RNA-Seq | 855.2 MB |
| U44_rectum_ulcerative_coliis_44_rectm_S22_L001_R1_001.fastq… | RNA-Seq | 834.3 MB |
| U44_rectum_ulcerative_coliis_44_rectm_S23_L001_R1_001.fastq… | RNA-Seq | 753.4 MB |
| U44_rectum_ulcerative_coliis_44_rectm_S24_L001_R1_001.fastq… | RNA-Seq | 612.2 MB |
| U45_pbmc_ulcerative_colitis_45_pBMC_S25_L001_R1_001.fastq.gz | RNA-Seq | 620.4 MB |
| U45_pbmc_ulcerative_colitis_45_pBMC_S26_L001_R1_001.fastq.gz | RNA-Seq | 797.3 MB |
| U45_pbmc_ulcerative_colitis_45_pBMC_S27_L001_R1_001.fastq.gz | RNA-Seq | 690.4 MB |
| U45_pbmc_ulcerative_colitis_45_pBMC_S28_L001_R1_001.fastq.gz | RNA-Seq | 841.9 MB |
| U45_R_ulcerative_colitis_45_rectum_S29_L002_R1_001.fastq.gz | RNA-Seq | 704.3 MB |
| U45_R_ulcerative_colitis_45_rectum_S30_L002_R1_001.fastq.gz | RNA-Seq | 802.6 MB |
| U45_R_ulcerative_colitis_45_rectum_S31_L002_R1_001.fastq.gz | RNA-Seq | 631.7 MB |
| U45_R_ulcerative_colitis_45_rectum_S32_L002_R1_001.fastq.gz | RNA-Seq | 865.2 MB |
| U4_pbmc_ulcerative_colitis_4_pbmc_S41_L002_R1_001.fastq.gz | RNA-Seq | 475.4 MB |
| U4_pbmc_ulcerative_colitis_4_pbmc_S42_L002_R1_001.fastq.gz | RNA-Seq | 911.6 MB |
| U4_pbmc_ulcerative_colitis_4_pbmc_S43_L002_R1_001.fastq.gz | RNA-Seq | 763.9 MB |
| U4_pbmc_ulcerative_colitis_4_pbmc_S44_L002_R1_001.fastq.gz | RNA-Seq | 507.1 MB |
| U4_rectum_ulcerative_4_rectum_S45_L002_R1_001.fastq.gz | RNA-Seq | 1.0 GB |
| U4_rectum_ulcerative_4_rectum_S46_L002_R1_001.fastq.gz | RNA-Seq | 517.8 MB |
| U4_rectum_ulcerative_4_rectum_S47_L002_R1_001.fastq.gz | RNA-Seq | 153.4 MB |
| U4_rectum_ulcerative_4_rectum_S48_L002_R1_001.fastq.gz | RNA-Seq | 970.3 MB |
| U5_pbmc_ulcerative_colitis_5_pbmc_S49_L002_R1_001.fastq.gz | RNA-Seq | 761.5 MB |
| U5_pbmc_ulcerative_colitis_5_pbmc_S50_L002_R1_001.fastq.gz | RNA-Seq | 464.0 MB |
| U5_pbmc_ulcerative_colitis_5_pbmc_S51_L002_R1_001.fastq.gz | RNA-Seq | 635.9 MB |
| U5_pbmc_ulcerative_colitis_5_pbmc_S52_L002_R1_001.fastq.gz | RNA-Seq | 521.4 MB |
| U5_rectum_ulcerative_colitis_rectum_S53_L002_R1_001.fastq.gz | RNA-Seq | 912.1 MB |
| U5_rectum_ulcerative_colitis_rectum_S54_L002_R1_001.fastq.gz | RNA-Seq | 319.5 MB |
| U5_rectum_ulcerative_colitis_rectum_S55_L002_R1_001.fastq.gz | RNA-Seq | 639.0 MB |
| U5_rectum_ulcerative_colitis_rectum_S56_L002_R1_001.fastq.gz | RNA-Seq | 611.9 MB |