Small intestine and colon tissue-resident memory CD8<sup>+</sup> T cells exhibit molecular heterogeneity and differential dependence on Eomes.
Abstract
Tissue-resident memory CD8<sup>+</sup> T (T<sub>RM</sub>) cells are a subset of memory T cells that play a critical role in limiting early pathogen spread and controlling infection. T<sub>RM</sub> cells exhibit differences across tissues, but their potential heterogeneity among distinct anatomic compartments within the small intestine and colon has not been well recognized. Here, by analyzing T<sub>RM</sub> cells from the lamina propria and epithelial compartments of the small intestine and colon, we showed that intestinal T<sub>RM</sub> cells exhibited distinctive patterns of cytokine and granzyme expression along with substantial transcriptional, epigenetic, and functional heterogeneity. The T-box transcription factor Eomes, which represses T<sub>RM</sub> cell formation in some tissues, exhibited unexpected context-specific regulatory roles in supporting the maintenance of established T<sub>RM</sub> cells in the small intestine, but not in the colon. Taken together, these data provide previously unappreciated insights into the heterogeneity and differential requirements for the formation vs. maintenance of intestinal T<sub>RM</sub> cells.
Publication Types
MeSH Terms
Funding
Linked Datasets (3)
Small intestine and colon tissue-resident memory CD8+ T cells exhibit transcriptional, epigenetic, and functional heterogeneity in concert with differential dependence on Eomesodermin [scATAC-seq]
Mus musculus5 data files
| File | Type | Size |
|---|---|---|
| 1_CIEL_S1_L001_R1_001.fastq.gz | ATAC-seq | 19.4 GB |
| 2_CLPL_S2_L001_R1_001.fastq.gz | ATAC-seq | 16.5 GB |
| 4_SI_IEL_S4_L001_R1_001.fastq.gz | ATAC-seq | 22.1 GB |
| 6_SI_LPL_S6_L001_R1_001.fastq.gz | ATAC-seq | 12.8 GB |
| SRR19560828.lite | ATAC-seq | 22.1 GB |
Small intestine and colon tissue-resident memory CD8+ T cells exhibit transcriptional, epigenetic, and functional heterogeneity in concert with differential dependence on Eomesodermin [CITE-seq]
Mus musculus16 data files
| File | Type | Size |
|---|---|---|
| 3_19_NE_CIEL_CD45_1_2_ADT_S68_L002_R1_001.fastq.gz | OTHER | 415.0 MB |
| 3_19_NE_CIEL_CD45_1_2_RNA_S68_L003_R1_001.fastq.gz | OTHER | 2.7 GB |
| 3_19_NE_CIEL_CD45_1_ADT_S67_L002_R1_001.fastq.gz | OTHER | 653.8 MB |
| 3_19_NE_CIEL_CD45_1_RNA_S67_L003_R1_001.fastq.gz | OTHER | 4.2 GB |
| 3_19_NE_CLPL_CD45_1_2_ADT_S66_L002_R1_001.fastq.gz | OTHER | 1.5 GB |
| 3_19_NE_CLPL_CD45_1_2_RNA_S66_L003_R1_001.fastq.gz | OTHER | 4.4 GB |
| 3_19_NE_CLPL_CD45_1_ADT_S65_L002_R1_001.fastq.gz | OTHER | 336.6 MB |
| 3_19_NE_CLPL_CD45_1_RNA_S65_L003_R1_001.fastq.gz | OTHER | 3.0 GB |
| 3_19_NE_SIIEL_CD45_1_2_ADT_S62_L002_R1_001.fastq.gz | OTHER | 3.9 GB |
| 3_19_NE_SIIEL_CD45_1_2_RNA_S62_L003_R1_001.fastq.gz | OTHER | 4.8 GB |
| 3_19_NE_SIIEL_CD45_1_ADT_S61_L002_R1_001.fastq.gz | OTHER | 5.2 GB |
| 3_19_NE_SIIEL_CD45_1_RNA_S61_L003_R1_001.fastq.gz | OTHER | 5.4 GB |
| 3_19_NE_SILPL_CD45_1_2_ADT_S64_L002_R1_001.fastq.gz | OTHER | 715.3 MB |
| 3_19_NE_SILPL_CD45_1_2_RNA_S64_L003_R1_001.fastq.gz | OTHER | 2.2 GB |
| 3_19_NE_SILPL_CD45_1_ADT_S63_L002_R1_001.fastq.gz | OTHER | 3.0 GB |
| 3_19_NE_SILPL_CD45_1_RNA_S63_L003_R1_001.fastq.gz | OTHER | 3.9 GB |
Potentially Related Datasets (2)
These accessions were text-mined from the PMC full text. They may be referenced for comparison, cited from other studies, or otherwise mentioned without being primary data for this paper.