A Small RNA-Catalytic Argonaute Pathway Tunes Germline Transcript Levels to Ensure Embryonic Divisions.
Adina Gerson-Gurwitz, Shaohe Wang, Shashank Sathe, Rebecca Green, Gene W Yeo, Karen Oegema, Arshad Desai
Cell · 2016 · Vol. 165 (2) · pp. 396-409
Abstract
Multiple division cycles without growth are a characteristic feature of early embryogenesis. The female germline loads proteins and RNAs into oocytes to support these divisions, which lack many quality control mechanisms operating in somatic cells undergoing growth. Here, we describe a small RNA-Argonaute pathway that ensures early embryonic divisions in C. elegans by employing catalytic slicing activity to broadly tune, instead of silence, germline gene expression. Misregulation of one target, a kinesin-13 microtubule depolymerase, underlies a major phenotype associated with pathway loss. Tuning of target transcript levels is guided by the density of homologous small RNAs, whose generation must ultimately be related to target sequence. Thus, the tuning action of a small RNA-catalytic Argonaute pathway generates oocytes capable of supporting embryogenesis. We speculate that the specialized nature of germline chromatin led to the emergence of small RNA-catalytic Argonaute pathways in the female germline as a post-transcriptional control layer to optimize oocyte composition.
Linked datasets 1
A Small RNA-Catalytic Argonaute Pathway Tunes Germline Transcript Levels to Ensure Embryonic Divisions
6239
136 data files
| File | Type | Size |
|---|---|---|
| AG-N2-control-5_S1_L008_R1_001.fastq.gz | RNA-Seq | 70.7 MB |
| AG-N2-control-5_S1_L008_R1_002.fastq.gz | RNA-Seq | 72.3 MB |
| AG-N2-control-5_S1_L008_R1_003.fastq.gz | RNA-Seq | 70.9 MB |
| AG-N2-control-5_S1_L008_R1_004.fastq.gz | RNA-Seq | 71.7 MB |
| AG-N2-control-5_S1_L008_R1_005.fastq.gz | RNA-Seq | 72.2 MB |
| AG-N2-control-5_S1_L008_R1_006.fastq.gz | RNA-Seq | 73.4 MB |
| AG-N2-control-5_S1_L008_R1_007.fastq.gz | RNA-Seq | 72.8 MB |
| AG-N2-control-5_S1_L008_R1_008.fastq.gz | RNA-Seq | 73.5 MB |
| AG-N2-control-5_S1_L008_R1_009.fastq.gz | RNA-Seq | 72.9 MB |
| AG-N2-control-5_S1_L008_R1_010.fastq.gz | RNA-Seq | 72.6 MB |
| AG-N2-control-5_S1_L008_R1_011.fastq.gz | RNA-Seq | 73.8 MB |
| AG-N2-control-5_S1_L008_R1_012.fastq.gz | RNA-Seq | 75.4 MB |
| AG-N2-control-5_S1_L008_R1_013.fastq.gz | RNA-Seq | 22.1 MB |
| AG-N2-control-6_S2_L008_R1_001.fastq.gz | RNA-Seq | 71.1 MB |
| AG-N2-control-6_S2_L008_R1_002.fastq.gz | RNA-Seq | 73.0 MB |
| AG-N2-control-6_S2_L008_R1_003.fastq.gz | RNA-Seq | 71.2 MB |
| AG-N2-control-6_S2_L008_R1_004.fastq.gz | RNA-Seq | 72.7 MB |
| AG-N2-control-6_S2_L008_R1_005.fastq.gz | RNA-Seq | 72.6 MB |
| AG-N2-control-6_S2_L008_R1_006.fastq.gz | RNA-Seq | 74.4 MB |
| AG-N2-control-6_S2_L008_R1_007.fastq.gz | RNA-Seq | 72.6 MB |
| AG-N2-control-6_S2_L008_R1_008.fastq.gz | RNA-Seq | 74.7 MB |
| AG-N2-control-6_S2_L008_R1_009.fastq.gz | RNA-Seq | 72.4 MB |
| AG-N2-control-6_S2_L008_R1_010.fastq.gz | RNA-Seq | 74.0 MB |
| AG-N2-control-6_S2_L008_R1_011.fastq.gz | RNA-Seq | 74.8 MB |
| AG-N2-control-6_S2_L008_R1_012.fastq.gz | RNA-Seq | 50.1 MB |
| AG-OD1175-SIN-1_S3_L008_R1_001.fastq.gz | RNA-Seq | 71.3 MB |
| AG-OD1175-SIN-1_S3_L008_R1_002.fastq.gz | RNA-Seq | 72.8 MB |
| AG-OD1175-SIN-1_S3_L008_R1_003.fastq.gz | RNA-Seq | 71.4 MB |
| AG-OD1175-SIN-1_S3_L008_R1_004.fastq.gz | RNA-Seq | 72.7 MB |
| AG-OD1175-SIN-1_S3_L008_R1_005.fastq.gz | RNA-Seq | 73.1 MB |
| AG-OD1175-SIN-1_S3_L008_R1_006.fastq.gz | RNA-Seq | 73.7 MB |
| AG-OD1175-SIN-1_S3_L008_R1_007.fastq.gz | RNA-Seq | 73.6 MB |
| AG-OD1175-SIN-1_S3_L008_R1_008.fastq.gz | RNA-Seq | 73.5 MB |
| AG-OD1175-SIN-1_S3_L008_R1_009.fastq.gz | RNA-Seq | 73.4 MB |
| AG-OD1175-SIN-1_S3_L008_R1_010.fastq.gz | RNA-Seq | 74.4 MB |
| AG-OD1175-SIN-1_S3_L008_R1_011.fastq.gz | RNA-Seq | 68.9 MB |
| AG-OD1175-SIN-2_S4_L008_R1_001.fastq.gz | RNA-Seq | 71.6 MB |
| AG-OD1175-SIN-2_S4_L008_R1_002.fastq.gz | RNA-Seq | 72.7 MB |
| AG-OD1175-SIN-2_S4_L008_R1_003.fastq.gz | RNA-Seq | 72.1 MB |
| AG-OD1175-SIN-2_S4_L008_R1_004.fastq.gz | RNA-Seq | 73.0 MB |
| AG-OD1175-SIN-2_S4_L008_R1_005.fastq.gz | RNA-Seq | 74.4 MB |
| AG-OD1175-SIN-2_S4_L008_R1_006.fastq.gz | RNA-Seq | 73.4 MB |
| AG-OD1175-SIN-2_S4_L008_R1_007.fastq.gz | RNA-Seq | 73.9 MB |
| AG-OD1175-SIN-2_S4_L008_R1_008.fastq.gz | RNA-Seq | 73.9 MB |
| AG-OD1175-SIN-2_S4_L008_R1_009.fastq.gz | RNA-Seq | 74.9 MB |
| AG-OD1175-SIN-2_S4_L008_R1_010.fastq.gz | RNA-Seq | 40.5 MB |
| AG-OD1463-WT-3_S5_L008_R1_001.fastq.gz | RNA-Seq | 71.1 MB |
| AG-OD1463-WT-3_S5_L008_R1_002.fastq.gz | RNA-Seq | 72.8 MB |
| AG-OD1463-WT-3_S5_L008_R1_003.fastq.gz | RNA-Seq | 71.1 MB |
| AG-OD1463-WT-3_S5_L008_R1_004.fastq.gz | RNA-Seq | 72.6 MB |
| AG-OD1463-WT-3_S5_L008_R1_005.fastq.gz | RNA-Seq | 72.5 MB |
| AG-OD1463-WT-3_S5_L008_R1_006.fastq.gz | RNA-Seq | 74.1 MB |
| AG-OD1463-WT-3_S5_L008_R1_007.fastq.gz | RNA-Seq | 72.8 MB |
| AG-OD1463-WT-3_S5_L008_R1_008.fastq.gz | RNA-Seq | 74.2 MB |
| AG-OD1463-WT-3_S5_L008_R1_009.fastq.gz | RNA-Seq | 72.6 MB |
| AG-OD1463-WT-3_S5_L008_R1_010.fastq.gz | RNA-Seq | 73.9 MB |
| AG-OD1463-WT-3_S5_L008_R1_011.fastq.gz | RNA-Seq | 75.3 MB |
| AG-OD1463-WT-3_S5_L008_R1_012.fastq.gz | RNA-Seq | 31.0 MB |
| AG-OD1463-WT-4_S6_L008_R1_001.fastq.gz | RNA-Seq | 71.8 MB |
| AG-OD1463-WT-4_S6_L008_R1_002.fastq.gz | RNA-Seq | 72.7 MB |
| AG-OD1463-WT-4_S6_L008_R1_003.fastq.gz | RNA-Seq | 72.4 MB |
| AG-OD1463-WT-4_S6_L008_R1_004.fastq.gz | RNA-Seq | 73.3 MB |
| AG-OD1463-WT-4_S6_L008_R1_005.fastq.gz | RNA-Seq | 74.3 MB |
| AG-OD1463-WT-4_S6_L008_R1_006.fastq.gz | RNA-Seq | 74.1 MB |
| AG-OD1463-WT-4_S6_L008_R1_007.fastq.gz | RNA-Seq | 73.4 MB |
| AG-OD1463-WT-4_S6_L008_R1_008.fastq.gz | RNA-Seq | 74.3 MB |
| AG-OD1463-WT-4_S6_L008_R1_009.fastq.gz | RNA-Seq | 76.1 MB |
| AG-OD1463-WT-4_S6_L008_R1_010.fastq.gz | RNA-Seq | 12.6 MB |
| SRR2922454_GSM1943675 | RNA-Seq | 70.0 MB |
| SRR2922455_GSM1943675 | RNA-Seq | 72.0 MB |
| SRR2922456_GSM1943675 | RNA-Seq | 73.0 MB |
| SRR2922457_GSM1943675 | RNA-Seq | 75.0 MB |
| SRR2922458_GSM1943675 | RNA-Seq | 22.0 MB |
| SRR2922459_GSM1943675 | RNA-Seq | 72.0 MB |
| SRR2922460_GSM1943675 | RNA-Seq | 70.0 MB |
| SRR2922461_GSM1943675 | RNA-Seq | 71.0 MB |
| SRR2922462_GSM1943675 | RNA-Seq | 72.0 MB |
| SRR2922463_GSM1943675 | RNA-Seq | 73.0 MB |
| SRR2922464_GSM1943675 | RNA-Seq | 72.0 MB |
| SRR2922465_GSM1943675 | RNA-Seq | 73.0 MB |
| SRR2922466_GSM1943675 | RNA-Seq | 72.0 MB |
| SRR2922467_GSM1943676 | RNA-Seq | 71.0 MB |
| SRR2922468_GSM1943676 | RNA-Seq | 74.0 MB |
| SRR2922469_GSM1943676 | RNA-Seq | 74.0 MB |
| SRR2922470_GSM1943676 | RNA-Seq | 50.0 MB |
| SRR2922471_GSM1943676 | RNA-Seq | 73.0 MB |
| SRR2922472_GSM1943676 | RNA-Seq | 71.0 MB |
| SRR2922473_GSM1943676 | RNA-Seq | 72.0 MB |
| SRR2922474_GSM1943676 | RNA-Seq | 72.0 MB |
| SRR2922475_GSM1943676 | RNA-Seq | 74.0 MB |
| SRR2922476_GSM1943676 | RNA-Seq | 72.0 MB |
| SRR2922477_GSM1943676 | RNA-Seq | 74.0 MB |
| SRR2922478_GSM1943676 | RNA-Seq | 72.0 MB |
| SRR2922479_GSM1943677 | RNA-Seq | 71.0 MB |
| SRR2922480_GSM1943677 | RNA-Seq | 73.0 MB |
| SRR2922481_GSM1943677 | RNA-Seq | 75.0 MB |
| SRR2922482_GSM1943677 | RNA-Seq | 30.0 MB |
| SRR2922483_GSM1943677 | RNA-Seq | 72.0 MB |
| SRR2922484_GSM1943677 | RNA-Seq | 71.0 MB |
| SRR2922485_GSM1943677 | RNA-Seq | 72.0 MB |
| SRR2922486_GSM1943677 | RNA-Seq | 72.0 MB |
| SRR2922487_GSM1943677 | RNA-Seq | 74.0 MB |
| SRR2922488_GSM1943677 | RNA-Seq | 72.0 MB |
| SRR2922489_GSM1943677 | RNA-Seq | 74.0 MB |
| SRR2922490_GSM1943677 | RNA-Seq | 72.0 MB |
| SRR2922491_GSM1943678 | RNA-Seq | 71.0 MB |
| SRR2922492_GSM1943678 | RNA-Seq | 12.0 MB |
| SRR2922493_GSM1943678 | RNA-Seq | 72.0 MB |
| SRR2922494_GSM1943678 | RNA-Seq | 72.0 MB |
| SRR2922495_GSM1943678 | RNA-Seq | 73.0 MB |
| SRR2922496_GSM1943678 | RNA-Seq | 74.0 MB |
| SRR2922497_GSM1943678 | RNA-Seq | 74.0 MB |
| SRR2922498_GSM1943678 | RNA-Seq | 73.0 MB |
| SRR2922499_GSM1943678 | RNA-Seq | 74.0 MB |
| SRR2922500_GSM1943678 | RNA-Seq | 76.0 MB |
| SRR2922501_GSM1943679 | RNA-Seq | 71.0 MB |
| SRR2922502_GSM1943679 | RNA-Seq | 74.0 MB |
| SRR2922503_GSM1943679 | RNA-Seq | 68.0 MB |
| SRR2922504_GSM1943679 | RNA-Seq | 72.0 MB |
| SRR2922505_GSM1943679 | RNA-Seq | 71.0 MB |
| SRR2922506_GSM1943679 | RNA-Seq | 72.0 MB |
| SRR2922507_GSM1943679 | RNA-Seq | 73.0 MB |
| SRR2922508_GSM1943679 | RNA-Seq | 73.0 MB |
| SRR2922509_GSM1943679 | RNA-Seq | 73.0 MB |
| SRR2922510_GSM1943679 | RNA-Seq | 73.0 MB |
| SRR2922511_GSM1943679 | RNA-Seq | 73.0 MB |
| SRR2922512_GSM1943680 | RNA-Seq | 71.0 MB |
| SRR2922513_GSM1943680 | RNA-Seq | 40.0 MB |
| SRR2922514_GSM1943680 | RNA-Seq | 72.0 MB |
| SRR2922515_GSM1943680 | RNA-Seq | 72.0 MB |
| SRR2922516_GSM1943680 | RNA-Seq | 73.0 MB |
| SRR2922517_GSM1943680 | RNA-Seq | 74.0 MB |
| SRR2922518_GSM1943680 | RNA-Seq | 73.0 MB |
| SRR2922519_GSM1943680 | RNA-Seq | 73.0 MB |
| SRR2922520_GSM1943680 | RNA-Seq | 73.0 MB |
| SRR2922521_GSM1943680 | RNA-Seq | 74.0 MB |
Potentially related datasets 1
These accessions were text-mined from the PMC full text. They may be referenced for comparison, cited from other studies, or otherwise mentioned without being primary data for this paper.
Analysis pipelines 1
| Pipeline | Assay | Steps | Dataset |
|---|---|---|---|
| GSE75128 Data Processing geo_data_processing | — | 7 | GSE75128 |
Funding 7 grants
| Grant | Funder |
|---|---|
| R01 HG004659 | NHGRI NIH HHS (United States) |
| R01 NS075449 | NINDS NIH HHS (United States) |
| HG004659 | NHGRI NIH HHS (United States) |
| NS075449 | NINDS NIH HHS (United States) |
| MH107369 | NIMH NIH HHS (United States) |
| R01 GM074215 | NIGMS NIH HHS (United States) |
| GM074215 | NIGMS NIH HHS (United States) |