A Small RNA-Catalytic Argonaute Pathway Tunes Germline Transcript Levels to Ensure Embryonic Divisions.
Adina Gerson-Gurwitz,
Shaohe Wang,
Shashank Sathe,
Rebecca Green,
Gene W Yeo,
Karen Oegema,
Arshad Desai
Cell
· 2016
· Vol. 165
(2)
· pp. 396-409
Abstract
Multiple division cycles without growth are a characteristic feature of early embryogenesis. The female germline loads proteins and RNAs into oocytes to support these divisions, which lack many quality control mechanisms operating in somatic cells undergoing growth. Here, we describe a small RNA-Argonaute pathway that ensures early embryonic divisions in C. elegans by employing catalytic slicing activity to broadly tune, instead of silence, germline gene expression. Misregulation of one target, a kinesin-13 microtubule depolymerase, underlies a major phenotype associated with pathway loss. Tuning of target transcript levels is guided by the density of homologous small RNAs, whose generation must ultimately be related to target sequence. Thus, the tuning action of a small RNA-catalytic Argonaute pathway generates oocytes capable of supporting embryogenesis. We speculate that the specialized nature of germline chromatin led to the emergence of small RNA-catalytic Argonaute pathways in the female germline as a post-transcriptional control layer to optimize oocyte composition.
Publication Types
["Journal Article", "Research Support, N.I.H., Extramural", "Research Support, Non-U.S. Gov't"]
MeSH Terms
["Animals", "Argonaute Proteins", "Base Sequence", "Caenorhabditis elegans", "Caenorhabditis elegans Proteins", "Cell Division", "Embryo, Nonmammalian", "Embryonic Development", "Female", "Kinesins", "Metabolic Networks and Pathways", "Microtubules", "Molecular Sequence Data", "Oocytes", "RNA Processing, Post-Transcriptional"]
Funding
R01 HG004659
NHGRI NIH HHS
(United States)
R01 NS075449
NINDS NIH HHS
(United States)
HG004659
NHGRI NIH HHS
(United States)
NS075449
NINDS NIH HHS
(United States)
MH107369
NIMH NIH HHS
(United States)
R01 GM074215
NIGMS NIH HHS
(United States)
GM074215
NIGMS NIH HHS
(United States)
Linked Datasets (1)
A Small RNA-Catalytic Argonaute Pathway Tunes Germline Transcript Levels to Ensure Embryonic Divisions
6239136 data files
| File | Type | Size |
|---|---|---|
| AG-N2-control-5_S1_L008_R1_001.fastq.gz | RNA-Seq | 70.7 MB |
| AG-N2-control-5_S1_L008_R1_001.fastq.gz | RNA-Seq | 70.7 MB |
| AG-N2-control-5_S1_L008_R1_002.fastq.gz | RNA-Seq | 72.3 MB |
| AG-N2-control-5_S1_L008_R1_002.fastq.gz | RNA-Seq | 72.3 MB |
| AG-N2-control-5_S1_L008_R1_003.fastq.gz | RNA-Seq | 70.9 MB |
| AG-N2-control-5_S1_L008_R1_003.fastq.gz | RNA-Seq | 70.9 MB |
| AG-N2-control-5_S1_L008_R1_004.fastq.gz | RNA-Seq | 71.7 MB |
| AG-N2-control-5_S1_L008_R1_004.fastq.gz | RNA-Seq | 71.7 MB |
| AG-N2-control-5_S1_L008_R1_005.fastq.gz | RNA-Seq | 72.2 MB |
| AG-N2-control-5_S1_L008_R1_005.fastq.gz | RNA-Seq | 72.2 MB |
| AG-N2-control-5_S1_L008_R1_006.fastq.gz | RNA-Seq | 73.4 MB |
| AG-N2-control-5_S1_L008_R1_006.fastq.gz | RNA-Seq | 73.4 MB |
| AG-N2-control-5_S1_L008_R1_007.fastq.gz | RNA-Seq | 72.8 MB |
| AG-N2-control-5_S1_L008_R1_007.fastq.gz | RNA-Seq | 72.8 MB |
| AG-N2-control-5_S1_L008_R1_008.fastq.gz | RNA-Seq | 73.5 MB |
| AG-N2-control-5_S1_L008_R1_008.fastq.gz | RNA-Seq | 73.5 MB |
| AG-N2-control-5_S1_L008_R1_009.fastq.gz | RNA-Seq | 72.9 MB |
| AG-N2-control-5_S1_L008_R1_009.fastq.gz | RNA-Seq | 72.9 MB |
| AG-N2-control-5_S1_L008_R1_010.fastq.gz | RNA-Seq | 72.6 MB |
| AG-N2-control-5_S1_L008_R1_010.fastq.gz | RNA-Seq | 72.6 MB |
| AG-N2-control-5_S1_L008_R1_011.fastq.gz | RNA-Seq | 73.8 MB |
| AG-N2-control-5_S1_L008_R1_011.fastq.gz | RNA-Seq | 73.8 MB |
| AG-N2-control-5_S1_L008_R1_012.fastq.gz | RNA-Seq | 75.4 MB |
| AG-N2-control-5_S1_L008_R1_012.fastq.gz | RNA-Seq | 75.4 MB |
| AG-N2-control-5_S1_L008_R1_013.fastq.gz | RNA-Seq | 22.1 MB |
| AG-N2-control-5_S1_L008_R1_013.fastq.gz | RNA-Seq | 22.1 MB |
| AG-N2-control-6_S2_L008_R1_001.fastq.gz | RNA-Seq | 71.1 MB |
| AG-N2-control-6_S2_L008_R1_001.fastq.gz | RNA-Seq | 71.1 MB |
| AG-N2-control-6_S2_L008_R1_002.fastq.gz | RNA-Seq | 73.0 MB |
| AG-N2-control-6_S2_L008_R1_002.fastq.gz | RNA-Seq | 73.0 MB |
| AG-N2-control-6_S2_L008_R1_003.fastq.gz | RNA-Seq | 71.2 MB |
| AG-N2-control-6_S2_L008_R1_003.fastq.gz | RNA-Seq | 71.2 MB |
| AG-N2-control-6_S2_L008_R1_004.fastq.gz | RNA-Seq | 72.7 MB |
| AG-N2-control-6_S2_L008_R1_004.fastq.gz | RNA-Seq | 72.7 MB |
| AG-N2-control-6_S2_L008_R1_005.fastq.gz | RNA-Seq | 72.6 MB |
| AG-N2-control-6_S2_L008_R1_005.fastq.gz | RNA-Seq | 72.6 MB |
| AG-N2-control-6_S2_L008_R1_006.fastq.gz | RNA-Seq | 74.4 MB |
| AG-N2-control-6_S2_L008_R1_006.fastq.gz | RNA-Seq | 74.4 MB |
| AG-N2-control-6_S2_L008_R1_007.fastq.gz | RNA-Seq | 72.6 MB |
| AG-N2-control-6_S2_L008_R1_007.fastq.gz | RNA-Seq | 72.6 MB |
| AG-N2-control-6_S2_L008_R1_008.fastq.gz | RNA-Seq | 74.7 MB |
| AG-N2-control-6_S2_L008_R1_008.fastq.gz | RNA-Seq | 74.7 MB |
| AG-N2-control-6_S2_L008_R1_009.fastq.gz | RNA-Seq | 72.4 MB |
| AG-N2-control-6_S2_L008_R1_009.fastq.gz | RNA-Seq | 72.4 MB |
| AG-N2-control-6_S2_L008_R1_010.fastq.gz | RNA-Seq | 74.0 MB |
| AG-N2-control-6_S2_L008_R1_010.fastq.gz | RNA-Seq | 74.0 MB |
| AG-N2-control-6_S2_L008_R1_011.fastq.gz | RNA-Seq | 74.8 MB |
| AG-N2-control-6_S2_L008_R1_011.fastq.gz | RNA-Seq | 74.8 MB |
| AG-N2-control-6_S2_L008_R1_012.fastq.gz | RNA-Seq | 50.1 MB |
| AG-N2-control-6_S2_L008_R1_012.fastq.gz | RNA-Seq | 50.1 MB |
| AG-OD1175-SIN-1_S3_L008_R1_001.fastq.gz | RNA-Seq | 71.3 MB |
| AG-OD1175-SIN-1_S3_L008_R1_001.fastq.gz | RNA-Seq | 71.3 MB |
| AG-OD1175-SIN-1_S3_L008_R1_002.fastq.gz | RNA-Seq | 72.8 MB |
| AG-OD1175-SIN-1_S3_L008_R1_002.fastq.gz | RNA-Seq | 72.8 MB |
| AG-OD1175-SIN-1_S3_L008_R1_003.fastq.gz | RNA-Seq | 71.4 MB |
| AG-OD1175-SIN-1_S3_L008_R1_003.fastq.gz | RNA-Seq | 71.4 MB |
| AG-OD1175-SIN-1_S3_L008_R1_004.fastq.gz | RNA-Seq | 72.7 MB |
| AG-OD1175-SIN-1_S3_L008_R1_004.fastq.gz | RNA-Seq | 72.7 MB |
| AG-OD1175-SIN-1_S3_L008_R1_005.fastq.gz | RNA-Seq | 73.1 MB |
| AG-OD1175-SIN-1_S3_L008_R1_005.fastq.gz | RNA-Seq | 73.1 MB |
| AG-OD1175-SIN-1_S3_L008_R1_006.fastq.gz | RNA-Seq | 73.7 MB |
| AG-OD1175-SIN-1_S3_L008_R1_006.fastq.gz | RNA-Seq | 73.7 MB |
| AG-OD1175-SIN-1_S3_L008_R1_007.fastq.gz | RNA-Seq | 73.6 MB |
| AG-OD1175-SIN-1_S3_L008_R1_007.fastq.gz | RNA-Seq | 73.6 MB |
| AG-OD1175-SIN-1_S3_L008_R1_008.fastq.gz | RNA-Seq | 73.5 MB |
| AG-OD1175-SIN-1_S3_L008_R1_008.fastq.gz | RNA-Seq | 73.5 MB |
| AG-OD1175-SIN-1_S3_L008_R1_009.fastq.gz | RNA-Seq | 73.4 MB |
| AG-OD1175-SIN-1_S3_L008_R1_009.fastq.gz | RNA-Seq | 73.4 MB |
| AG-OD1175-SIN-1_S3_L008_R1_010.fastq.gz | RNA-Seq | 74.4 MB |
| AG-OD1175-SIN-1_S3_L008_R1_010.fastq.gz | RNA-Seq | 74.4 MB |
| AG-OD1175-SIN-1_S3_L008_R1_011.fastq.gz | RNA-Seq | 68.9 MB |
| AG-OD1175-SIN-1_S3_L008_R1_011.fastq.gz | RNA-Seq | 68.9 MB |
| AG-OD1175-SIN-2_S4_L008_R1_001.fastq.gz | RNA-Seq | 71.6 MB |
| AG-OD1175-SIN-2_S4_L008_R1_001.fastq.gz | RNA-Seq | 71.6 MB |
| AG-OD1175-SIN-2_S4_L008_R1_002.fastq.gz | RNA-Seq | 72.7 MB |
| AG-OD1175-SIN-2_S4_L008_R1_002.fastq.gz | RNA-Seq | 72.7 MB |
| AG-OD1175-SIN-2_S4_L008_R1_003.fastq.gz | RNA-Seq | 72.1 MB |
| AG-OD1175-SIN-2_S4_L008_R1_003.fastq.gz | RNA-Seq | 72.1 MB |
| AG-OD1175-SIN-2_S4_L008_R1_004.fastq.gz | RNA-Seq | 73.0 MB |
| AG-OD1175-SIN-2_S4_L008_R1_004.fastq.gz | RNA-Seq | 73.0 MB |
| AG-OD1175-SIN-2_S4_L008_R1_005.fastq.gz | RNA-Seq | 74.4 MB |
| AG-OD1175-SIN-2_S4_L008_R1_005.fastq.gz | RNA-Seq | 74.4 MB |
| AG-OD1175-SIN-2_S4_L008_R1_006.fastq.gz | RNA-Seq | 73.4 MB |
| AG-OD1175-SIN-2_S4_L008_R1_006.fastq.gz | RNA-Seq | 73.4 MB |
| AG-OD1175-SIN-2_S4_L008_R1_007.fastq.gz | RNA-Seq | 73.9 MB |
| AG-OD1175-SIN-2_S4_L008_R1_007.fastq.gz | RNA-Seq | 73.9 MB |
| AG-OD1175-SIN-2_S4_L008_R1_008.fastq.gz | RNA-Seq | 73.9 MB |
| AG-OD1175-SIN-2_S4_L008_R1_008.fastq.gz | RNA-Seq | 73.9 MB |
| AG-OD1175-SIN-2_S4_L008_R1_009.fastq.gz | RNA-Seq | 74.9 MB |
| AG-OD1175-SIN-2_S4_L008_R1_009.fastq.gz | RNA-Seq | 74.9 MB |
| AG-OD1175-SIN-2_S4_L008_R1_010.fastq.gz | RNA-Seq | 40.5 MB |
| AG-OD1175-SIN-2_S4_L008_R1_010.fastq.gz | RNA-Seq | 40.5 MB |
| AG-OD1463-WT-3_S5_L008_R1_001.fastq.gz | RNA-Seq | 71.1 MB |
| AG-OD1463-WT-3_S5_L008_R1_001.fastq.gz | RNA-Seq | 71.1 MB |
| AG-OD1463-WT-3_S5_L008_R1_002.fastq.gz | RNA-Seq | 72.8 MB |
| AG-OD1463-WT-3_S5_L008_R1_002.fastq.gz | RNA-Seq | 72.8 MB |
| AG-OD1463-WT-3_S5_L008_R1_003.fastq.gz | RNA-Seq | 71.1 MB |
| AG-OD1463-WT-3_S5_L008_R1_003.fastq.gz | RNA-Seq | 71.1 MB |
| AG-OD1463-WT-3_S5_L008_R1_004.fastq.gz | RNA-Seq | 72.6 MB |
| AG-OD1463-WT-3_S5_L008_R1_004.fastq.gz | RNA-Seq | 72.6 MB |
| AG-OD1463-WT-3_S5_L008_R1_005.fastq.gz | RNA-Seq | 72.5 MB |
| AG-OD1463-WT-3_S5_L008_R1_005.fastq.gz | RNA-Seq | 72.5 MB |
| AG-OD1463-WT-3_S5_L008_R1_006.fastq.gz | RNA-Seq | 74.1 MB |
| AG-OD1463-WT-3_S5_L008_R1_006.fastq.gz | RNA-Seq | 74.1 MB |
| AG-OD1463-WT-3_S5_L008_R1_007.fastq.gz | RNA-Seq | 72.8 MB |
| AG-OD1463-WT-3_S5_L008_R1_007.fastq.gz | RNA-Seq | 72.8 MB |
| AG-OD1463-WT-3_S5_L008_R1_008.fastq.gz | RNA-Seq | 74.2 MB |
| AG-OD1463-WT-3_S5_L008_R1_008.fastq.gz | RNA-Seq | 74.2 MB |
| AG-OD1463-WT-3_S5_L008_R1_009.fastq.gz | RNA-Seq | 72.6 MB |
| AG-OD1463-WT-3_S5_L008_R1_009.fastq.gz | RNA-Seq | 72.6 MB |
| AG-OD1463-WT-3_S5_L008_R1_010.fastq.gz | RNA-Seq | 73.9 MB |
| AG-OD1463-WT-3_S5_L008_R1_010.fastq.gz | RNA-Seq | 73.9 MB |
| AG-OD1463-WT-3_S5_L008_R1_011.fastq.gz | RNA-Seq | 75.3 MB |
| AG-OD1463-WT-3_S5_L008_R1_011.fastq.gz | RNA-Seq | 75.3 MB |
| AG-OD1463-WT-3_S5_L008_R1_012.fastq.gz | RNA-Seq | 31.0 MB |
| AG-OD1463-WT-3_S5_L008_R1_012.fastq.gz | RNA-Seq | 31.0 MB |
| AG-OD1463-WT-4_S6_L008_R1_001.fastq.gz | RNA-Seq | 71.8 MB |
| AG-OD1463-WT-4_S6_L008_R1_001.fastq.gz | RNA-Seq | 71.8 MB |
| AG-OD1463-WT-4_S6_L008_R1_002.fastq.gz | RNA-Seq | 72.7 MB |
| AG-OD1463-WT-4_S6_L008_R1_002.fastq.gz | RNA-Seq | 72.7 MB |
| AG-OD1463-WT-4_S6_L008_R1_003.fastq.gz | RNA-Seq | 72.4 MB |
| AG-OD1463-WT-4_S6_L008_R1_003.fastq.gz | RNA-Seq | 72.4 MB |
| AG-OD1463-WT-4_S6_L008_R1_004.fastq.gz | RNA-Seq | 73.3 MB |
| AG-OD1463-WT-4_S6_L008_R1_004.fastq.gz | RNA-Seq | 73.3 MB |
| AG-OD1463-WT-4_S6_L008_R1_005.fastq.gz | RNA-Seq | 74.3 MB |
| AG-OD1463-WT-4_S6_L008_R1_005.fastq.gz | RNA-Seq | 74.3 MB |
| AG-OD1463-WT-4_S6_L008_R1_006.fastq.gz | RNA-Seq | 74.1 MB |
| AG-OD1463-WT-4_S6_L008_R1_006.fastq.gz | RNA-Seq | 74.1 MB |
| AG-OD1463-WT-4_S6_L008_R1_007.fastq.gz | RNA-Seq | 73.4 MB |
| AG-OD1463-WT-4_S6_L008_R1_007.fastq.gz | RNA-Seq | 73.4 MB |
| AG-OD1463-WT-4_S6_L008_R1_008.fastq.gz | RNA-Seq | 74.3 MB |
| AG-OD1463-WT-4_S6_L008_R1_008.fastq.gz | RNA-Seq | 74.3 MB |
| AG-OD1463-WT-4_S6_L008_R1_009.fastq.gz | RNA-Seq | 76.1 MB |
| AG-OD1463-WT-4_S6_L008_R1_009.fastq.gz | RNA-Seq | 76.1 MB |
| AG-OD1463-WT-4_S6_L008_R1_010.fastq.gz | RNA-Seq | 12.6 MB |
| AG-OD1463-WT-4_S6_L008_R1_010.fastq.gz | RNA-Seq | 12.6 MB |
Analysis Pipelines (1)
GSE75128 Data Processing
7 steps
geo_data_processing
GSE75128