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5 results for “CRISPR-Cas”
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A phage nucleus-associated RNA-binding protein is required for jumbo phage infection.
Nucleic acids research · 2024 · 52(8): 4440-4455
Large-genome bacteriophages (jumbo phages) of the proposed family Chimalliviridae assemble a nucleus-like compartment bounded by a protein shell that protects the replicating phage genome from host-encoded restriction enzymes and DNA-targeting CRISPR-Cas nucleases. While the nuclear shell provides broad protection against …
PMID 38554115 DOI ↗
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Crosstalk between CRISPR-Cas9 and the human transcriptome.
Nature communications · 2022 · 13(1): 1125
CRISPR-Cas9 expression independent of its cognate synthetic guide RNA (gRNA) causes widespread genomic DNA damage in human cells. To investigate whether Cas9 can interact with endogenous human RNA transcripts independent of its guide, we perform eCLIP (enhanced CLIP) of Cas9 …
PMID 35236841 DOI ↗
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Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA Editing.
Cell reports · 2020 · 33(5): 108350
Site-directed RNA editing approaches offer great potential to correct genetic mutations in somatic cells while avoiding permanent off-target genomic edits. Nuclease-dead RNA-targeting CRISPR-Cas systems recruit functional effectors to RNA molecules in a programmable fashion. Here, we demonstrate a Streptococcus pyogenes …
PMID 33147453 DOI ↗
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RNA-targeting CRISPR systems from metagenomic discovery to transcriptomic engineering.
Nature cell biology · 2020 · 22(2): 143-150
Deployment of RNA-guided DNA endonuclease CRISPR-Cas technology has led to radical advances in biology. As the functional diversity of CRISPR-Cas and parallel systems is further explored, RNA manipulation is emerging as a powerful mode of CRISPR-based engineering. In this Perspective, …
PMID 32015437 DOI ↗
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Applications of Cas9 as an RNA-programmed RNA-binding protein.
BioEssays : news and reviews in molecular, cellular and developmental biology · 2015 · 37(7): 732-9
The Streptococcus pyogenes CRISPR-Cas system has gained widespread application as a genome editing and gene regulation tool as simultaneous cellular delivery of the Cas9 protein and guide RNAs enables recognition of specific DNA sequences. The recent discovery that Cas9 can …
PMID 25880497 DOI ↗
Datasets 10
Investigating m6A-relevant gene function in neural progenitor cells using CRISPR-Cas9 genome editing and RNA-seq
Homo sapiens · GPL24676
Inhibition of YTHDF2 triggers proteotoxic cell death in MYC-driven breast cancer
Homo sapiens · GPL20301
Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease
Homo sapiens · GPL15520
In vivo CRISPR screening unveils RNA binding protein dependencies for leukemic stem cells and identifies ELAVL1 as a potential therapeutic target [eCLIPseq]
Mus musculus · GPL21103
In vivo CRISPR screening unveils RNA binding protein dependencies for leukemic stem cells and identifies ELAVL1 as a potential therapeutic target [RNA-seq]
Mus musculus · GPL18480
RNA-Targeting CRISPR/Cas13d System Alleviates Disease-Related Phenotypes in Pre-clinical Models of Huntingtonâs Disease (Human).
Homo sapiens · GPL18573
Crosstalk between CRISPR-Cas9 and the human transcriptome
Homo sapiens · GPL20301
A two-step in vivo CRISPR screen unveils pervasive RNA binding protein dependencies for leukemic stem cells and identifies ELAVL1 as a therapeutic target
Homo sapiens · GPL18460
A role for alternative splicing in circadian control of insulin secretion and glucose homeostasis
Mus musculus · GPL19057
RNA-Targeting CRISPR/Cas13d System Alleviates Disease-Related Phenotypes in Pre-clinical Models of Huntingtonâs Disease (Mouse).
Mus musculus · GPL19057
Analyses 6
GSE214108 · 3 steps · RNA-Seq · code_examples
An RNA-targeting CRISPR-Cas13d system alleviates disease-related phenotypes in Huntington's disease models. (2023)
GSE214109 · 5 steps · RNA-Seq · code_examples
An RNA-targeting CRISPR-Cas13d system alleviates disease-related phenotypes in Huntington's disease models. (2023)
GSE214110 · 3 steps · GSE · code_examples
An RNA-targeting CRISPR-Cas13d system alleviates disease-related phenotypes in Huntington's disease models. (2023)
GSE167466 · 18 steps · RIP-Seq · code_examples
Crosstalk between CRISPR-Cas9 and the human transcriptome. (2022)
GSE55887 · 5 steps · ChIP-Seq · code_examples
Crosstalk between CRISPR-Cas9 and the human transcriptome. (2022)
GSE152684 · 8 steps · RNA-Seq · code_examples
Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA Editing. (2020)