DDX5 promotes oncogene C3 and FABP1 expressions and drives intestinal inflammation and tumorigenesis.
Nazia Abbasi, Tianyun Long, Yuxin Li, Brian A Yee, Benjamin S Cho, Juan E Hernandez, Evelyn Ma, Parth R Patel, Debashis Sahoo, Ibrahim M Sayed, Nissi Varki, Soumita Das, Pradipta Ghosh, Gene W Yeo, Wendy Jia Men Huang
Life science alliance · 2020 · Vol. 3 (10)
Abstract
Tumorigenesis in different segments of the intestinal tract involves tissue-specific oncogenic drivers. In the colon, complement component 3 (C3) activation is a major contributor to inflammation and malignancies. By contrast, tumorigenesis in the small intestine involves fatty acid-binding protein 1 (FABP1). However, little is known of the upstream mechanisms driving their expressions in different segments of the intestinal tract. Here, we report that the RNA-binding protein DDX5 binds to the mRNA transcripts of <i>C3</i> and <i>Fabp1</i> to augment their expressions posttranscriptionally. Knocking out DDX5 in epithelial cells protected mice from intestinal tumorigenesis and dextran sodium sulfate (DSS)-induced colitis. Identification of DDX5 as a common upstream regulator of tissue-specific oncogenic molecules provides an excellent therapeutic target for intestinal diseases.
Linked datasets 2
DDX5 promotes oncogene C3 and FABP1 expressions and drives intestinal inflammation and tumorigenesis
Mus musculus
16 data files
| File | Type | Size |
|---|---|---|
| NA01_S1_L008_R1_001.fastq.gz | RNA-Seq | 3.7 GB |
| NA02_S2_L008_R1_001.fastq.gz | RNA-Seq | 1.7 GB |
| NA03_S3_L008_R1_001.fastq.gz | RNA-Seq | 2.4 GB |
| NA04_S4_L008_R1_001.fastq.gz | RNA-Seq | 2.8 GB |
| NA05_S5_L008_R1_001.fastq.gz | RNA-Seq | 2.8 GB |
| NA06_S6_L008_R1_001.fastq.gz | RNA-Seq | 3.0 GB |
| NA07_S7_L008_R1_001.fastq.gz | RNA-Seq | 3.2 GB |
| NA08_S8_L008_R1_001.fastq.gz | RNA-Seq | 2.9 GB |
| SRR8320977_GSM3515090 | RNA-Seq | 3.7 GB |
| SRR8320978_GSM3515091 | RNA-Seq | 2.4 GB |
| SRR8320979_GSM3515092 | RNA-Seq | 1.7 GB |
| SRR8320980_GSM3515093 | RNA-Seq | 2.8 GB |
| SRR8320981_GSM3515094 | RNA-Seq | 2.8 GB |
| SRR8320982_GSM3515095 | RNA-Seq | 3.2 GB |
| SRR8320983_GSM3515096 | RNA-Seq | 3.0 GB |
| SRR8320984_GSM3515097 | RNA-Seq | 2.9 GB |
DDX5 targets tissue specific RNAs to promote intestine tumorigenesis
Mus musculus
24 data files
| File | Type | Size |
|---|---|---|
| INPUT_G1_S36_L003_R1_001.fastq | RIP-Seq | 1.5 GB |
| INPUT_G2_S38_L003_R1_001.fastq | RIP-Seq | 2.0 GB |
| IP_G1_S37_L003_R1_001.fastq | RIP-Seq | 1.5 GB |
| IP_G2_S39_L003_R1_001.fastq | RIP-Seq | 736.2 MB |
| mAb_IN_1_Ep_S35_L003_R1_001.fastq.gz | RIP-Seq | 550.5 MB |
| mAb_IN_2_Ep_S37_L003_R1_001.fastq.gz | RIP-Seq | 695.1 MB |
| mAb_IP_1_Ep_S36_L003_R1_001.fastq.gz | RIP-Seq | 746.7 MB |
| mAb_IP_2_Ep_S38_L003_R1_001.fastq.gz | RIP-Seq | 533.2 MB |
| rAb_IN_1_Ep_S39_L003_R1_001.fastq.gz | RIP-Seq | 631.1 MB |
| rAb_IN_2_Ep_S41_L003_R1_001.fastq.gz | RIP-Seq | 623.4 MB |
| rAb_IP_1_Ep_S40_L003_R1_001.fastq.gz | RIP-Seq | 457.5 MB |
| rAb_IP_2_Ep_S42_L003_R1_001.fastq.gz | RIP-Seq | 547.6 MB |
| SRR11590535_GSM4491125 | RIP-Seq | 1.5 GB |
| SRR11590536_GSM4491126 | RIP-Seq | 1.5 GB |
| SRR11590537_GSM4491127 | RIP-Seq | 736.0 MB |
| SRR11590538_GSM4491128 | RIP-Seq | 2.0 GB |
| SRR11591014_GSM4491238 | RIP-Seq | 746.0 MB |
| SRR11591015_GSM4491238 | RIP-Seq | 533.0 MB |
| SRR11591016_GSM4491239 | RIP-Seq | 550.0 MB |
| SRR11591017_GSM4491239 | RIP-Seq | 695.0 MB |
| SRR11591018_GSM4491240 | RIP-Seq | 457.0 MB |
| SRR11591019_GSM4491240 | RIP-Seq | 547.0 MB |
| SRR11591020_GSM4491241 | RIP-Seq | 631.0 MB |
| SRR11591021_GSM4491241 | RIP-Seq | 623.0 MB |
Potentially related datasets 11
These accessions were text-mined from the PMC full text. They may be referenced for comparison, cited from other studies, or otherwise mentioned without being primary data for this paper.
| Accession | Source | Title |
|---|---|---|
| GSE17538 | GSE · GEO GEO ↗ | Experimentally Derived Metastasis Gene Expression Profile Predicts Recurrence and Death in Colon Cancer Patients |
| GSE17536 | GSE · GEO GEO ↗ | Metastasis Gene Expression Profile Predicts Recurrence and Death in Colon Cancer Patients (Moffitt Samples) |
| GSE31595 | GSE · GEO GEO ↗ | Gene Expression Profiles in Stage II and III Colon Cancer. Application of a 128-gene signature |
| GSE87211 | GSE · GEO GEO ↗ | Colorectal cancer susceptibility loci as predictive markers of rectal cancer prognosis after surgery |
| GSE13067 | GSE · GEO GEO ↗ | Expression data from primary colorectal cancers |
| GSE14333 | GSE · GEO GEO ↗ | Expression data from 290 primary colorectal cancers |
| GSE37892 | GSE · GEO GEO ↗ | A seven-gene signature aggregates a subgroup of stage II colon cancers with stage III. |
| GSE33113 | GSE · GEO GEO ↗ | AMC colon cancer AJCCII |
| GSE5851 | GSE · GEO GEO ↗ | Phase II exploratory pharmacogenomics study of cetuximab monotherapy in patients with advanced metastatic CRC |
| GSE123881 | GSE · GEO GEO ↗ | DDX5 promotes oncogene C3 and FABP1 expressions and drives intestinal inflammation and tumorigenesis |
| GSE124023 | GSE · GEO GEO ↗ | DDX5 targets tissue specific RNAs to promote intestine tumorigenesis |
Analysis pipelines 11
| Pipeline | Assay | Steps | Dataset |
|---|---|---|---|
| GSE123881 Data Processing geo_data_processing | — | 3 | GSE123881 |
| GSE124023 eCLIP Data Processing geo_data_processing | eCLIP | 22 | GSE124023 |
| GSE13067 Data Processing geo_data_processing | — | 3 | GSE13067 |
| GSE14333 Microarray Data Processing geo_data_processing | Microarray | 2 | GSE14333 |
| GSE17536 Microarray Data Processing geo_data_processing | Microarray | 3 | GSE17536 |
| GSE17538 Data Processing geo_data_processing | — | 3 | GSE17538 |
| GSE31595 Microarray Data Processing geo_data_processing | Microarray | 2 | GSE31595 |
| GSE33113 Microarray Data Processing geo_data_processing | Microarray | 1 | GSE33113 |
| GSE37892 Data Processing geo_data_processing | — | 3 | GSE37892 |
| GSE5851 Data Processing geo_data_processing | — | 1 | GSE5851 |
| GSE87211 Microarray Data Processing geo_data_processing | Microarray | 1 | GSE87211 |
Funding 15 grants
| Grant | Funder |
|---|---|
| S10 OD026929 | NIH HHS (United States) |
| R01 HG004659 | NHGRI NIH HHS (United States) |
| P30 CA023100 | NCI NIH HHS (United States) |
| T32 CA067754 | NCI NIH HHS (United States) |
| U41 HG009889 | NHGRI NIH HHS (United States) |
| R01 GM124494 | NIGMS NIH HHS (United States) |
| R01 CA238042 | NCI NIH HHS (United States) |
| R01 CA100768 | NCI NIH HHS (United States) |
| UG3 TR003355 | NCATS NIH HHS (United States) |
| R01 DK107585 | NIDDK NIH HHS (United States) |
| R01 AI141630 | NIAID NIH HHS (United States) |
| UG3 TR002968 | NCATS NIH HHS (United States) |
| UH3 TR002968 | NCATS NIH HHS (United States) |
| R01 GM138385 | NIGMS NIH HHS (United States) |
| R00 CA151673 | NCI NIH HHS (United States) |